抗CD137单克隆抗体对哮喘患者外周血CD4^＋CD25^＋T淋巴细胞死亡方式的影响

目的探讨抗CD啪单克隆抗体对哮喘患者外周血CD4^＋CD25^＋T淋巴细胞的影响。方法采用密度梯度离心法及尼龙棉柱法分离16例健康志愿者（对照组）及12例哮喘患者（哮喘组）外周血T淋巴细胞，磁性细胞分离器（MACS）分离得到CD4^＋CD25^＋T淋巴细胞，分别利用电镜及流式细胞仪观察、检测抗CD137单克隆抗体干预72h的细胞自噬率、凋亡率、胀亡率及FOXp3的表达。结果抗CD137单克隆抗体干预后两组外周血CD4^＋CD25^＋T淋巴细胞自噬率及凋亡率均增加，但哮喘组均低于对照组。结论抗CD137单克隆抗体可促进CD4^＋CD25^＋T淋巴细胞凋亡和自噬。

Effects of anti-CD137 mAb on the death patterns of peripheral blood CD+4CD+25T lymphocytes

Objective To observe the effects of anti-CD137 monoclonal antibody in peripheral blood CD4^＋CD25^＋T lymphocytes in patiems with asthma. Methods Peripheral blood T lymphocyte of sixteen healthy adults （ contorl group） and twelve asthma patients （ asthma group） were separated by Pereoll and harvested by using nylon column. Then, CD4^＋CD25^＋T lymphocytes were isolated by MACS（ magnetic cell sorting）. The autophagy, apoptosis, oncosis and FOXp3 rate of the cells treated by anti-CD137 mAb for 72 h were analyzed by electron microscope and flow eytometry. Results The autophagy and apoptosis rate of peripheral CD4^＋CD25^＋T lymphocytes in both group increased after-treated by anti-CD137 mAb, but the effect was less in the asthma group than that in the control group（P 〈0.05 ）. Conclusion Anti-CD137 mAb help treat asthma by increasing the autophagy and apoptosis rate of peripheral blood CD4^＋CD25^＋T lymphocytes.