葡萄籽原花青素对海马细胞氧化损伤和凋亡的影响

目的:观察葡萄籽原花青素(GSP)对大鼠海马细胞氧化损伤的保护作用,及对凋亡相关基因Bcl-2和Bax表达的影响。方法:建立H2O2致海马细胞损伤模型,观察GSP对细胞形态和细胞存活率的影响;并用免疫组化法检测GSP对凋亡相关基因Bcl-2与Bax蛋白表达的影响。结果:1 mmol/L H2O2诱导海马细胞损伤,细胞活力下降(P<0.001),细胞凋亡小体明显增多,细胞中Bcl-2表达下降和Bax表达升高。而GSP可明显减少细胞损伤,上调细胞中Bcl-2的表达,使细胞中Bax表达明显减少,(P<0.01),细胞凋亡明显改善。结论:GSP对H2O2诱导的海马神经细胞凋亡具有保护作用,与GSP可增加Bcl-2表达同时减少Bax表达有关。

Effects of Grape Seed Proanthocyanidin on Oxidative Induced Damage and Apoptosis in Rat Hippocampal Neurons

Objective: To investigate effects of grape seed proanthocyanidin(GSP) on oxidative induced damage,and the expression of apoptosis related genes Bcl-2 and Bax in hippocampal neurons.Methods: H2O2 induced hippocampal neurons damage models were established.Effects of GSP on cell morphology,apoptosis and cell viability were observed.Immunohistochemical method was used to study the effect of GSP on expression of apoptosis related genes Bcl-2 and Bax.Results: H2O2 significantly decreased cell viability and Bcl-2 expression,and increased the number of apoptotic bodies and expression of Bcl-2 and Bax.GSP could obviously antagonise the H2O2 induced effects.Conclusion: GSP has protective effects on H2O2-induced apoptosis in hippocampal neurons,maybe through increasing Bcl-2 expression and decreasing Bax expression simultaneously.