Granuloma pouch assay |
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Authors: | Gerhard Zbinden Peter Maier Susan Alder |
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Institution: | (1) Institute of Toxicology, Swiss Federal Institute of Technology and University of Zurich, Schorenstrasse 16, CH-8603 Schwerzenbach, Switzerland |
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Abstract: | Proliferation of granulation tissue on the inside of a subcutaneous (s.c.) air pocket was induced in rats by administration
of 0.5 ml of 0.25% croton oil (granuloma pouch). Two days after induction of the granuloma, i.e., during the period of maximal
cell growth, a single dose of 0.6 mg or 0.1 mg N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) was administered into the pouch.
Fibrosarcomas of various histopathological types developed in 87% of the rats receiving the high dose and in 64% of the low
dose animals. The mean latency period was 47.5 and 51.7 weeks respectively. Only one local sarcoma developed in rats treated
with 0.6 mg MNNG by the s.c. route, and no tumors were observed in the groups treated with 0.1 mg s.c. The appearance of local
sarcomas in the granuloma pouch tissue is correlated with previously reported frequency of point mutations (OUAR and HGPRT−) induced in granuloma fibroblasts with the same doses of MNNG. Possible mechanisms explaining the marked enhancement of the
carcinogenic effect of MNNG in the granuloma pouch assay are discussed. |
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Keywords: | Granuloma pouch assay MNNG Short-term carcinogenicity tests |
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