通脉益智胶囊对脑缺血再灌注损伤诱导的小鼠海马神经细胞凋亡的影响

目的　观察通脉益智胶囊对脑缺血再灌注损伤诱导的小鼠海马神经细胞凋亡的影响 ,探讨其神经保护作用的机制。方法　雄性ICR小鼠 4 0只 ,随机分为 4组 :假手术组、模型组、通脉益智胶囊组、西药 (喜德镇 )对照组。建立小鼠反复脑缺血再灌注模型。分别采用光学显微镜、透射电子显微镜观察 ,原位末端标记法 (TUNEL)染色并计算凋亡指数 (ApoptosisIndexAI)。结果　通脉益智胶囊组病理损害明显减轻 ,凋亡指数显著低于模型组及西药对照组。结论　通脉益智胶囊可显著抑制由脑缺血再灌注损伤诱导的小鼠海马神经细胞凋亡 ,从而起到一定程度的神经保护作用

Effects of Tongmaiyizhi Capsules on Apoptosis of Mouse Hippocampal Neurocytes Induced by the Injury due to Ischemia-reperfusion

Objective To observe the effects of Tongmaiyizhi Capsules (TC) on apoptosis of mouse hippocampal neurocytes induced by the injury due to ischemia reperfusion and investigate the mechanism of the nerve protective effects of TC. Methods 40 Male ICR mice were randomly divided into the sham operation group, model group, TC group and hydergine control group. A mouse model of repeated cerebral ischemia and reperfusion was established for the experiment. The optical microscope and transmission electron microscope were used to observe apoptosis of mouse hippocampal neurocytes. TUNEL labeling was used to label the apoptotic neurocytes and then the apoptotic index was calculated. Results The pathologic injuries were markedly reduced in the TC group, and the apoptotic index in the TC group was remarkably lower than that in either the model group or the hydergine control group. Conclusion TC can markedly inhibit the apoptosis of mouse hippocampal neurocytes induced by the injury due to cerebral ischemia and reperfusion, hence, exerting certain nerve protective effects.