强力霉素对胃癌细胞增殖及Notch1、 MMP-9表达的影响 |
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引用本文: | 许 敏 李 红. 强力霉素对胃癌细胞增殖及Notch1、 MMP-9表达的影响[J]. 天津医药, 2014, 42(6): 533-535 |
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作者姓名: | 许 敏 李 红 |
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作者单位: | 辽宁医学院 |
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摘 要: | 【摘要】目的 研究强力霉素对胃癌细胞BGC-823细胞的增殖及 Notch1、MMP-9 蛋白表达的影响,为胃癌的治疗提供新的抗肿瘤药物。方法 分别以 0、5、10、20、40 μg/mL 终浓度的强力霉素作用于人胃癌细胞系 BGC-823,运用 MTT 法检测细胞增殖程度;流式细胞仪检测细胞周期分布的影响; Western Blot 检测 Notch1、MMP-9 蛋白水平的表达。结果 强力霉素能够抑制人胃癌细胞BGC-823细胞的增殖,且与药物的剂量及作用时间呈正相关(P<0.01);强力霉素能够改变胃癌细胞BGC-823周期的分布,随着浓度的增加,S 期所占比例降低(P<0.01);并且随着浓度的增加,Notch1 的表达增强,MMP-9 的表达降低(P<0.01)。结论 强力霉素能够抑制胃癌细胞BGC-823的增殖,Notch1上调为可能的机制之一
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关 键 词: | 胃癌细胞 强力霉素 Notch1 MMP-9 细胞增殖 细胞周期 |
收稿时间: | 2013-11-08 |
修稿时间: | 2014-02-17 |
Doxycycline on gastric cancer cell proliferation and Notch1,MMP-9 expression |
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Abstract: | 【Abstract】: Objective: Doxycycline on gastric cancer cell line BGC-823 proliferation and Notch1,MMP-9 expression, in order to provide a new new anticancer drugs. Method: Respectively 0, 5, 10, 20, 40 μg/mL final concentration of doxycycline effects on human gastric cancer cell line BGC-823.Cell proliferation was detected was detected by MTT degree; flow cytometry cell cycle distribution; Western Blot detection Notch1, MMP-9 protein expression. Results: Doxycycline can inhibit the proliferation of human gastric cancer cells line BGC-823, and the effect of drug dose and time-dependent(P<0.01); doxycycline alter gastric cancer cell line BGC-823 cycle distribution, with increasing concentration, S the proportion of lower phase(P<0.01); as the concentration increase, Notch1 expression enhancement, MMP-9 expression decreased (P<0.01). Conclusion: Doxycyclinecan inhibit gastric cancer cell line BGC-823 proliferation, Notch1 up as one of the possible mechanisms. |
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Keywords: | gastric cancer cells doxycycline Notch1 MMP-9 cell proliferation cell cycle |
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