小鼠腹腔巨噬细胞膜结合IL－1和TNF活性研究

本文实验提示小鼠腹腔巨噬细胞（Ｍ）经体内或体外ＬＰＳ／ＳｉＯ２刺激后，其固定的完整细胞膜或细胞膜粗提物具有ＩＬＩ和ＴＮＦ活性。在１ｈ、１２ｈ两种刺激时间内，膜结合ＩＬ－１活性无显著变化，但膜结合ＴＮＦ活性则随刺激时间延长而有所降低。刺激后的Ｍ在经ｐＨ３．０的甘氨酸盐酸处理后，膜结合ＩＬ－１和膜结合ＴＮＦ活性均有下降，以ＩＬ－１活性下降较为明显。ＩＬ－１受体拮抗剂能完全阻断膜结合ＩＬ－１活性，对膜结合ＴＮＦ活性亦有轻度的抑制效应，提示在膜ＴＮＦ发挥作用过程中，膜结合ＩＬ－１可能也参与了这一过程。

MEMBRANE-ASSOCIATED INTERLEUKIN-1 AND TUMOR NECROSIS FACTOR ON ACTIVATED MURINE PERITONEAL MACROPHAGES

The membrane-associated interleukin-1(mIL-1)and tumor necrosis factor(mTNF)were examined either onthe surface of intact murine peritoneal macrophages or on the partially purified membranes of macrophages,The re-sults indicated that the activities of mIL-1 and mTNF were increased on the silicon or lipopolysaccharide(LPS)chal-lenged macrophages. Expression kinetics analyses indicated that the mIL-1 was constant on the macrophages thathad been challenged for 1 or 12 hours,but the mTNF was attenuated gradually as the elongation of challenge timeon macrophages. The partially decrease of mIL-1 and mTNF activities was observed on the macrophages that hadbeen incubated in low pH buffer (glycine-HCl buffer ,pH0)for 20 minutes and washed vigorously with the samebuffer, and the decrease of IL-1 activity was comparatively prominent. Meanwhile,the activity of mIL-1 can beblocked totally by IL-1 receptor antagonist (IL-1RA),but the activity of mTNF is only partially reduced by IL-1RA.