环氧化酶-2在腹部肠管火器伤后肝组织中的表达及意义

目的:探讨环氧化酶-2(COX-2)在腹部肠管火器伤后肝组织中表达的变化及其意义。方法:健康长白仔猪42头随机等分为对照组和腹部肠管火器伤后1、2、4、8、12和24 h实验组。建立腹部肠管火器伤模型后,用免疫组化图像分析法测定各组肝组织COX-2的表达,同时测定血清ALT、AST水平,在光镜下观察各组肝脏组织学变化。结果:伤后各组肝组织COX-2表达明显高于对照组(P<0.05),并于伤后2 h和12 h出现2个高峰。伤后各组出现逐渐加重的肝细胞水肿、变性、坏死,血清ALT、AST水平在伤后也明显升高,也于伤后2 h和12 h出现2个高峰。相关分析表明,伤后肝组织COX-2与血清ALT、AST水平均呈正相关(r值分别为0.850、0.757,P<0.05)。结论:腹部肠管火器伤后肝组织COX-2表达增强,与肝脏结构和功能损伤基本一致,COX-2在腹部肠管火器伤后继发性肝损伤中具有重要意义。

Expression of COX-2 in liver tissues after intestinal firearm wound and its significance

Objective:To investigate the expression of COX-2 and its significance in liver tissues after intestinal firearm wound.Methods: A total of 42 Chang-Bai piglets were randomized into 7 groups: control group and wounded 1 h,2 h,4 h,8 h,12 h and 24 h group.The models of intestinal firearm wound were established.Hepatic COX-2 expression was measured with immunohistochemical staining and image analysis in all groups.Levels of serum ALT and AST were also determined.The changes of hepatic tissues were observed under light microscope in all groups.Results:Levels of hepatic COX-2 expression in wounded groups were significantly elevated compared to that of control group(P<0.05),and there were two peaks in 2 h group and 12 h group.Hepatocyte edema,metamorphosis and necrosis were gradually aggravated from 1 h group to 24 h group.Levels of serum ALT and AST were all markedly increased,and there were two peaks in 2 h group and 12 h group(P<0.05).In addition,the level of hepatic COX-2 was positively correlated with serum ALT and AST levels(r=0.850,0.757,P<0.05).Conclusion:After intestinal firearm wound,the expression of hepatic COX-2 is increased and positively correlated with hepatic morphological and functional changes.The COX-2 may play important roles in subsequent liver injury after the wound.