用猴的类DPB基因诱导的PCR指纹图对HLA-DP基因快速相容性测定

目的 将PCR指纹图用于个体间HLA－DP的基因水平交叉配型，确定供－受体间DPB基因的相容性。方法 引入猴的类DPB等位基因的扩增产物作通用性异源双链生成物（UHG），使原不有产生POCR指纹图的DPB基因扩增产物在非变性PAGE中显示特定的“卫星条带”，从而可用于供受体间HLA－DP基因的相容性测定。结果 11株和HLA纯合的B淋巴细胞系和随机个体进行PCR－DPB指纹图及交叉配型分析，能敏感地反映了个体间DP基因的差异，特别是在纯合子DPB＊0201和杂合子DPB＊0402／0501中初步发现了可能存在着未能被PCR－RFLP区分的新的亚型或新的等位基因，但是尚不能区分DPB＊0201和DPB＊0402两个等位基因，有待今后进一步探索。结论 初步表明该技术可有效判别供－受体DBP基因匹配性，并有简单、快速等优点。

A study on HLA-DP gene crossmatching test with PCR-fingerprinting by introducing a monkey′s DPB-like gene

Objective　To determinate HLA-DPB gene compatibility between donors and recipients by HLA-DPB gene crossmatching with PCR-fingerprinting. Methods　By introducing a PCR product of monkey′s DPB-like gene as a universal heteroduplex generator (UHG), each PCR product of HLA-DPB allele that does not generate heteroduplexes originally in the non-denatured PAGE can form some specific “satellite” bands which are used to determine the compatibility of HLA-DP allele by crossmatching with PCR-fingerprinting. Results　The study shows in HLA either 11 homozygous BLCLs or heterozygous individuals, the crossmatching analysis with PCR-fingerprinting can distinguish the HLA-DP differences between individuals sensitively and also find some challenging results primarily in the homozygous DPB*0201 BLCLs and heterozygous DPB*0402/0501 individuals. However the difference between DPB*0201 allele and DPB*0402 allele is still distinguished. Conclusion　PCR-fingerprinting is simple and rapid method in determining the HLA-DP compatibility between donors and recipients at genetic level.