Gene expression profile differences in high and low metastatic human ovarian c ancer cell lines by gene chip

Objectives To study the difference between gene expressions of high (H0- 8 910PM) and low (HO- 891 0) metastatic human ovarian carcinoma cell lines and screen novel associated gen es by cDNA microarray.Methods cDNA retro- transcribed from equal quantities of mRNA derived from high and low metastatic tumor cells or normal ovarian tissues were labeled with Cy5 and Cy3 f luorescein as probes.The mixed probe was hybridized with two pieces of BioDoo r 4096 double dot human whole gene chip and scanned with a ScanArray 3000 laser scanner.The acquired image was analyzed by ImaGene 3.0 software. Results A total of 355 genes with expression lev els more than 3 times larger were found by comparing the HO- 8910 cell with norm al ovarian epithelial cells.A total of 323 genes with expression levels more t han 3 times larger in HO- 8910PM cells compared to normal ovarian epithelium cel ls were also detected.A total of 165 genes whose expression levels were more t han two times those of HO- 8910PM cells compared to their mother cell line ( HO- 8910) were detected. Twenty- one genes with expression levels >3 times wer e found from comparison of these two tumor cell lines.Conclusions cDNA microarray techniques are effective in screening differential gene express ion between two human ovarian cancer cell lines (H0- 8910PM; HO- 8910) and norma l ovarian epithelial cells.These genes may be related to the genesis and devel opment of ovarian carcinoma. Analysis of the human ovarian cancer gene express ion profile with cDNA microarray may help in gene diagnosis, treatment and preve ntion.