生物衍生组织工程骨支架材料的制备及理化特性

我们用物理化学方法处理自制的天然生物衍生骨支架材料 ,测试其理化性质及力学强度 ,为骨组织工程研究提供可供选择的支架材料。将猪肋骨进行一系列理化处理 ,制成完全脱蛋白骨 (FDB)、部分脱蛋白骨(PDPB)、部分脱钙骨 (PDCB)三种材料 ,用扫描电镜观察其形貌特征 ,用 X射线衍射分析、X射线能量散射分析材料成份 ,用微量凯氏定氮法测定蛋白质含量 ,并对材料的力学性能进行分析测定。结果发现 ,FDB、PDPB、PDCB三种材料皆保持原骨组织的天然网状孔隙系统。其钙 /磷比值依次为 1.81、1.74和 1.5 0。三种材料的蛋白质含量为0 .0 1%± 0 .0 2 % ,2 2 .41%± 0 .83%和 35 .75 %± 2 .2 1% ,力学强度大小为 :PDCB>PDPB>FDB。可见 ,用不同理化方法处理制得的三种生物衍生骨材料皆保存原骨组织的天然网状孔隙系统 ,所含有机及无机成分含量不同 ,力学性能亦有差异 ,尚需要进一步验证其细胞相容性和组织相容性。

Experimental Studies on Procedures and Properties of Natural Derived Scaffold Materials of Tissue Engineered Bone

To detect the properties of natural xenogeneic bone derived materials which were processed with different physical and chemical treatments, we made fully deproteinized bone(FDB), partially deproteinized bone (PDPB), partially decalcified bone(PDCB) from pig ribs. Their morphological features, constitute components and mechanical properties were examined by scanning electron microscopy, x-rays diffraction analysis, mechanical assay and so on. The results showed that FDB, PDPB and PDCB maintained natural network pore system. The ratios of calcium to phosphorus were 1.81, 1.74 and 1.50, and the protein contents were 0.01% +/- 0.02%, 22.41% +/- 0.83% and 35.75% +/- 2.12% respectively. The sequence of their mechanic strength was PDCB > PDPB > FDB. These data indicate that FDB, PDPB and PDCB possess natural network pore system. Their organic and inorganic component ratios and contents are different, so their mechanic properties are not alike. Additionally, more investigations will be necessary to detect the biocompatibility of the three different scaffold materials of natural derived bone.