Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
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| Institution: | 1. Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;2. Animal House Unit, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;3. Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand;4. Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan;5. Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Japan;1. Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Brazil;2. Virginia Tech Carilion School of Medicine and Research Institute, Roanoke, VA, USA;3. Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA;1. Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Saint Kitts and Nevis, West Indies;2. Department of Hygiene, Sapporo Medical University, Sapporo, Hokkaido, Japan;3. Behavioral Science Foundation, Saint Kitts and Nevis, West Indies;4. Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India |
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| Abstract: | Whole genomes of G9P19] human (RVA/Human-wt/THA/CMH-S070-13/2013/G9P19]) and porcine (RVA/Pig-wt/THA/CMP-015-12/2012/G9P19]) rotaviruses concurrently detected in the same geographical area in northern Thailand were sequenced and analyzed for their genetic relationships using bioinformatic tools. The complete genome sequence of human rotavirus RVA/Human-wt/THA/CMH-S070-13/2013/G9P19] was most closely related to those of porcine rotavirus RVA/Pig-wt/THA/CMP-015-12/2012/G9P19] and to those of porcine-like human and porcine rotaviruses reference strains than to those of human rotavirus reference strains. The genotype constellation of G9P19] detected in human and piglet were identical and displayed as the G9-P19]-I5-R1-C1-M1-A8-N1-T1-E1-H1 genotypes with the nucleotide sequence identities of VP7, VP4, VP6, VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4, and NSP5 at 99.0%, 99.5%, 93.2%, 97.7%, 97.7%, 85.6%, 89.5%, 93.2%, 92.9%, 94.0%, and 98.1%, respectively. The findings indicate that human rotavirus strain RVA/Human-wt/THA/CMH-S070-13/2013/G9P19] containing the genome segments of porcine genetic backbone is most likely a human rotavirus of porcine origin. Our data provide an evidence of interspecies transmission and whole-genome transmission of nonreassorted G9P19] porcine RVA to human occurring in nature in northern Thailand. |
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