Changes in electroencephalographic characteristics and blood-brain barrier permeability in WAG/Rij rats with cortical dysplasia |
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| Institution: | 1. Department of Physiology, Medical Faculty of Kocaeli University, Kocaeli, Turkey;2. Department of Laboratory Animals Science, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey;3. Department of Neuroscience, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey;4. Department of Forensic Medicine, Istanbul Faculty of Medicine, Istanbul University, Turkey;5. Koç University School of Medicine, Department of Physiology, Rumelifeneri Yolu, Sar?yer, 34450 Istanbul, Turkey;6. Department of Neurology, Istanbul Faculty of Medicine, Istanbul University, Turkey;7. Department of Histology and Embryology, Istanbul Faculty of Medicine, Istanbul University, Turkey;1. Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel;2. Casali Institute of Applied Chemistry, Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, Israel;3. Department of Surgery, Drexel University College of Medicine, Philadelphia, PA, USA;4. Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA, USA;5. Department of Neurology, Agnes Ginges Center for Human Neurogenetics, Hadassah-Hebrew University Medical Center, Jerusalem, Israel;6. Department of Radiology, Children''s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, USA;7. Department of Radiology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel;8. The David R. Bloom Centre for Pharmacy and Dr. Adolf and Klara Brettler Centre for Research in Molecular Pharmacology and Therapeutics, The Hebrew University of Jerusalem, Jerusalem, Israel;1. Department of Neurophysiology and Chronobiology, Institute of Zoology, Jagiellonian University in Krakow, Krakow, Poland;2. Faculty of Chemistry, Jagiellonian University in Krakow, Krakow, Poland;1. Student Research Committee, Babol University of Medical Sciences, Babol, Mazandaran, Iran;2. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Mazandaran, Iran;3. Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Mazandaran, Iran;4. Nanochemistry Research Lab, Faculty of Chemistry, University of Mazandaran, Babolsar, Mazandaran, Iran;5. Department of Pharmacology, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Mazandaran, Iran;1. Karadeniz Technical University, Faculty of Medicine, Neurology Department, Clinical Neurophysiology Unit, Trabzon, Turkey;2. Karadeniz Technical University, Faculty of Medicine, Physiology Department, Trabzon, Turkey;3. University of Health Sciences, Faculty of Medicine, Medical Physiology Department, Istanbul, Turkey |
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| Abstract: | PurposeThis study investigated the effects of cortical dysplasia (CD) on electrophysiology and blood-brain barrier (BBB) permeability in WAG/Rij rats with genetic absence epilepsy.MethodsPregnant WAG/Rij rats were exposed to 145 cGy of gamma-irradiation on embryonic day 17 to induce CD. An electroencephalogram was recorded from cortices subdurally in the offspring of the pregnant animals. Horseradish peroxidase (HRP) was used as determinant of BBB permeability.ResultsA massive tissue loss in the cerebral cortex was seen in WAG/Rij rats with CD (p < 0.05). There was a significant decrease in the number and duration of spike-and-wave discharges (SWDs) and an increase in the frequency of SWDs in the WAG/Rij rats with CD when compared with the properties of SWDs in intact WAG/Rij rats (p < 0.01). Ultrastructurally, the accumulation of HRP reaction products in the cerebral cortex and thalamus of WAG/Rij rats was significantly higher than that of control values (p < 0.01). The accumulation of HRP reaction products in the cerebral cortex and thalamus regions of WAG/Rij rats with CD increased and was higher than that of the control and WAG/Rij animals (p < 0.01).ConclusionIn our study, we showed that number and duration of SWDs decreased and SWD frequency increased in WAG/Rij rats with CD, suggesting a shift in seizure pattern. The association of these alterations with significant loss of cortical thickness and increased BBB permeability to HRP tracer may represent a causal relation of the EEG abnormalities with cerebral structural changes in these animals. |
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