Subtype analysis of zoonotic pathogen Cryptosporidium skunk genotype |
| |
| Institution: | 1. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA;2. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan 471023, China;3. New York City Department of Environmental Protection, Bureau of Water Supply, Division of Water Quality Science and Research, Valhalla, New York, USA;5. Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China;6. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;1. Department of Food and Environmental Virology, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland;2. Cryptosporidium Reference Unit, Public Health Wales Microbiology, Singleton Hospital, Swansea SA2 8QA, UK;3. Department of Parasitology and Invasive Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland;1. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, Australia;2. Pathology Queensland, Townsville Hospital, Townsville, Queensland, Australia;3. School of Medical and Applied Sciences, Central Queensland University, Rockhampton, North Rockhampton, Queensland, Australia;4. Melbourne Water Corporation, Victoria, Australia;5. Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria;1. Parasitology Service, National Centre for Microbiology, Carlos III Health Institute, Majadahonda, Madrid, Spain;2. Microbiology Service, University Hospital Severo Ochoa, Leganés, Madrid, Spain;3. Microbiology and Clinical Parasitology Service, University Hospital Puerta de Hierro, Majadahonda, Madrid, Spain |
| |
| Abstract: | Cryptosporidium skunk genotype is a zoonotic pathogen commonly identified in surface water. Thus far, no subtyping tool exists for characterizing its transmission in humans and animals and transport in environment. In this study, a subtyping tool based on the 60 kDa glycoprotein (gp60) gene previously developed for Cryptosporidium chipmunk genotype I was used in the characterization of Cryptosporidium skunk genotype in animal and storm runoff samples from a watershed in New York. Altogether, 17 positive samples from this watershed and 5 human and animal specimens from other areas were analyzed. We identified 14 subtypes of Cryptosporidium skunk genotype, 11 of which were seen in the watershed. In phylogenetic analysis, these subtypes belonged to 4 subtype families (XVIa, XVIb, XVIc, and XVId). No host-adapted subtypes were identified and the two subtypes in humans were genetically similar to some in raccoons, otters, and storm runoff samples from the watershed. The characteristics of gp60 protein sequences of the Cryptosporidium skunk genotype are similar to those of other Cryptosporidium species, but only its XVIb subtype family has a putative furin cleavage site. This subtyping tool might be useful in characterizing Cryptosporidium skunk genotype in clinical and environmental samples. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
