ALA-PDT suppressing the cell growth and reducing the lipogenesis in human SZ95 sebocytes by mTOR signaling pathway in vitro |
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Affiliation: | 1. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea;2. Oriental BioMed Lab, Daejeon, Republic of Korea;3. Oriental Medical College of Daejeon University, Daejeon, Republic of Korea;4. Department of Chemical & Biological Engineering, Hanbat National University, Daejeon, Republic of Korea;5. Skin Med Company, Daejeon, Republic of Korea;1. Department of Dermatology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 160 Pujian Road, Pudong Area, 200127, Shanghai, P.R. China;2. Division of Endocrinology and Metabolism, Department of Internal Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 160 Pujian Road, Pudong Area, 200127, Shanghai, P.R. China;3. Department of Dermatology, Goztepe Research and Training Hospital, School of Medicine, Istanbul Medeniyet University, Fahrettin Kerim Gökay cad., 34722, Goztepe, Kadıkoy, Istanbul, Turkey;4. Department of Dermatology, Rumaillah Hospital, Hamad Medical Cooperation, Doha, Katar;5. IZZ-Immunologie Zentrum Zürich, Walchestr. 11, CH 8006, Zürich, Switzerland;6. Department of Dermatology and Allergy, Technische Universität München, Biedersteinerstr. 29, D-80802, Munich, Germany |
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Abstract: | Background5-Aminolevulinic acid mediated −photodynamic therapy (ALA-PDT) is known to be effective in treating acne vulgaris and other sebaceous gland-related diseases. However, the therapeutic mechanisms of ALA-PDT still remain undetermined. In this study, we aimed to investigate the effects and mechanisms of ALA-PDT on the cell growth and lipogenesis of human SZ95 sebocytes.Material and methodsHuman SZ95 sebocytes were treated with different concentration of ALA-PDT.CCK-8 assay was used to detect cell proliferation activity. Fluorescence microscope and flow cytometry were used to observe the secretion of lipids in SZ95 cells after Nile red staining. Western blotting was used to detect and analyze the protein expression level of P-p70 S6 K/p70 S6 K, P-4E-BP1/4E-BP1, SREBP-1, PPARγ, P-mTOR/mTOR, and P-Raptor/Raptor. Mean while, mTOR pathway activator IGF-1 and mTORC1 inhibitor rapamycin were added to observe the interferences on the ALA-PDT treatment of SZ95 cells.ResultsALA-PDT suppressed the cell growth and reduced the secretion of lipids in a dose-dependent manner in SZ95 cells. ALA-PDT reduced the protein levels of P-p70 S6 K (T389), SREBP-1, PPARγ, P-mTOR and P-Raptor. IGF-1 had counter effects on ALA-PDT, and rapamycin enhanced the effects of ALA-PDT in SZ95 cells in suppressing the cell growth and reducing the secretion of lipids.ConclusionALA-PDT suppressed the cell growth in SZ95 cells by mTOR-p70 S6K(T389) signaling and reduced the lipogenesis in SZ95 cells by mTOR-SREBP-1/PPARγ signaling. Sebaceous glands atrophy and reduction of sebum secretion after ALA-PDT may be caused by the suppression of lipogenesis and cell growth in sebocytes. |
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Keywords: | Sebocytes Lipogenesis mTOR 5-Aminolevulinic acid Photodynamic therapy |
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