Interaction of cholera toxin B subunit with T and B lymphocytes |
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| Institution: | 1. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Science Avenue, 6, Pushchino, Moscow Region 142290, Russian Federation;2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya street, 16/10, Moscow, GSP-7, 117997, Russian Federation;1. Laboratório de Ultraestrutura, Instituto Aggeu Magalhães – FIOCRUZ, Recife, Brazil;2. Programa de Pós-graduação em Ciências Biológicas, Centro de Biociências, Universidade Federal de Pernambuco - UFPE, Recife, Brazil;3. Laboratório de Síntese e Vetorização de Moléculas (LSVM), Universidade Estadual da Paraíba, João Pessoa, Brazil;4. Programa de Pós-graduação em Biotecnologia/RENORBIO, Universidade Federal de Pernambuco - UFPE, Recife, Brazil;5. Laboratório de Biologia Celular e Ultraestrutura, Centro de Tecnologias Estratégicas do Nordeste – CETENE, Recife, Brazil;1. Center for Research and Education on Drug Discovery, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan;2. Laboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan;1. Medical Pharmacology Department, Faculty of Medicine, Cairo University, Egypt;2. Department of Clinical Pathology, National Cancer Institute, Cairo University, Egypt;1. Laboratory of Cardiovascular Diseases, Guangdong Medical University, Zhanjiang 524001, People''s Republic of China;2. Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, People''s Republic of China;3. Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, People''s Republic of China;4. Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, People''s Republic of China;5. Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan 523808, People''s Republic of China;6. The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, People''s Republic of China |
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| Abstract: | We have prepared 125I-labeled cholera toxin B subunit (125I-labeled CT-B, a specific activity of 98 Ci/mmol) and found that its binding to T and B lymphocytes from the blood of healthy donors was high-affinity (Kd 2.8 and 3.0 nM, respectively). The binding of labeled protein was completely inhibited by unlabeled thymosin-α1 (TM-α1), interferon-α2 (IFN-α2), and the synthetic peptide LKEKK that corresponds to residues 16–20 in TM-α1 and 131–135 in IFN-α2, but was not inhibited by the synthetic peptide KKEKL with inverted amino acid sequence (Ki > 10 μM). Thus, TM-α1, IFN-α2, and the peptide: LKEKK bind with high affinity and specificity to CT-B receptor on donor blood T and B lymphocytes. It was found that CT-B and the peptide: LKEKK at concentrations of 10–1000 nM increased in a dose-dependent manner the soluble guanylate cyclase activity in T and B lymphocytes. |
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