Effect of methylene blue-induced photodynamic therapy on a Streptococcus mutans biofilm model |
| |
| Institution: | 1. Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto – University of São Paulo, Avenida do Café s/n, Ribeirão Preto, SP 14040-904, Brazil;2. Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café s/n, Ribeirão Preto, SP CEP 14040-903, Brazil;3. Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto – University of São Paulo, Avenida do Café s/n, Ribeirão Preto, SP 14040-904, Brazil;1. Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, 605 014, India;2. Department of Physics, School of Physical, Chemical & Applied Sciences, Pondicherry University, Puducherry, 605014, India;3. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia;1. Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran;2. Department of Chemical and Petroleum Engineering, Faculty of Science, Sharif University of Technology, Tehran, Iran;1. Department of Microbiology, Hospital Universitario Miguel Servet, Zaragoza, Spain;2. University of Zaragoza, Zaragoza, Spain;3. Department of Optic, Ensenada Center for Scientific Research and Higher Education (CICESE), Baja California, Mexico;4. Department of Microbiology, University of Sevilla, Sevilla, Spain;5. IIS Aragón, Zaragoza, Spain;6. Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain;7. Department of Dermatology, Hospital San Jorge, Huesca, Spain;1. Department of Microbiology, School of Medicine, International Campus, Tehran University of Medical Sciences (IC-TUMS), Tehran, Iran;2. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;3. Laser Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran;4. Private Practice, Tehran, Iran;5. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran;6. Innovative Research Center, Islamic Azad University, Mashhad Branch, Mashhad, Iran;7. Pediatrics Infectious Disease Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;8. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran;1. Dental Research Center, São Leopoldo Mandic, Campinas, Brazil;2. Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil;3. School of Dentistry, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil;4. Center for Lasers and Applications, Nuclear and Energy Research Institute IPEN-CNEN/SP, São Paulo, Brazil;5. Department of Biomedical Engineering, ABC Federal University (UFABC), Santo Andre, SP, Brazil |
| |
| Abstract: | BackgroundSeveral studies have reported the use of antimicrobial photodynamic therapy (aPDT) to control biofilm but its efficacy depends on several factors, such as biofilm model used. This study aims to examine whether exposure to diode laser combined with methylene blue affects the bacterial viability and polysaccharide content in a Streptococcus mutans cariogenic biofilm model, which simulated ‘feast-famine’ episodes of exposure to sucrose that occur in the oral cavity.Materials and methodsS. mutans biofilms were formed on acrylic resin discs and exposed to a 10% sucrose solution for 1 min, eight times/day. After growing for 48 h, the biofilms were submitted to the following treatments, twice daily (n = 4): (i) 0.9% NaCl (NaCl) as the negative control; (ii) 0.12% chlorhexidine digluconate (CHX) as the positive antibacterial control; (iii) diode laser combined with methylene blue, using an energy density of 320 J/cm2 (aPDT). After 120 h of growth, the biofilm formed on each disc was collected to determine the viable bacterial counts and concentration of insoluble exopolysaccharides (IEPS) and intracellular polysaccharides (IPS).ResultsBacterial counts in the biofilms formed differed among the treatments. Compared with NaCl, aPDT significantly destabilized biofilm (p < 0.0001). aPDT and CHX equally lowered the concentration of IEPS and IPS in biofilms.ConclusionUnder the experimental conditions assessed, our findings indicate that a twice-daily treatment with diode laser combined with methylene blue effectively decreased bacterial viability and the intra- and extracellular polysaccharide concentration in biofilms of S. mutans, a cariogenic bacterium. |
| |
| Keywords: | Biofilm Diode laser Methylene blue Polysaccharides |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
