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| 补阳还五汤预先干预对大鼠脑死亡后心肺炎症细胞因子表达的抑制作用 | |
| 引用本文： | 陈洁,曾耀英,曾慧兰,刘嘉雯,苏泽轩,郝彤彤. 补阳还五汤预先干预对大鼠脑死亡后心肺炎症细胞因子表达的抑制作用[J]. 中草药, 2010, 41(5): 757-761 |
| 作者姓名： | 陈洁 曾耀英 曾慧兰 刘嘉雯 苏泽轩 郝彤彤 |
| 作者单位： | 1. 暨南大学附属第一医院泌尿外科/肾移植科,广东广州,510630 2. 暨南大学组织移植与免疫中心广东广州,510632 3. 暨南大学附属第一医院血液科,广东广州,510630 |
| 基金项目： | 教育部留学回国人员科研启动基金资助项目,教育部科学技术研究重点项目,国家中医药管理局留学回国人员科技活动择优资助项目 |
| 摘 要： | 目的观察补阳还五汤预先干预大鼠抑制其脑死亡后心、肺炎症细胞因子表达。方法雄性Wistar大鼠30只,体质量180~200g,随机分3组。对照组:正常大鼠麻醉后取心和肺;脑死亡模型组:诱导大鼠脑死亡;补阳还五汤组:脑死亡诱导前7d,连续每天1次给大鼠ig补阳还五汤水煎剂(1.8mL/100g,12.6g/kg)。大鼠脑死亡诱导成功,机械呼吸6h,若平均动脉压大于80mmHg,则取其心和肺。RT-PCR检测心、肺组织肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)mRNA表达,Western blotting检测心、肺组织TNF-α和IL-1β蛋白及磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)蛋白表达。结果模型组心肺TNF-α、IL-1βmRNA和蛋白表达及p-p38MAPK蛋白表达比对照组显著增加(P0.01)。补阳还五汤组各指标比模型组显著降低(P0.05),但仍比对照组显著增加(P0.01)。结论补阳还五汤预先干预大鼠能显著抑制其脑死亡后心肺炎症细胞因子表达,可能与其在不同层面阻断p38MAPK信号通路关键靶点有关。 |
| 关 键 词： | 补阳还五汤 脑死亡 炎症细胞因子 p38丝裂原活化蛋白激酶 |
| 收稿时间： | 2009-09-29 |
Inhibition on inflammatory cytokine expressions in heart and lung from brain death rats pretreated with Buyang Huanwu Decoction |
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| CHEN Jie,ZENG Yao-ying,ZENG Hui-lan,LIU Jia-wen,SU Ze-xuan and HAO Tong-tong. Inhibition on inflammatory cytokine expressions in heart and lung from brain death rats pretreated with Buyang Huanwu Decoction[J]. Chinese Traditional and Herbal Drugs, 2010, 41(5): 757-761 | |
| Authors: | CHEN Jie ZENG Yao-ying ZENG Hui-lan LIU Jia-wen SU Ze-xuan HAO Tong-tong |
| Affiliation: | Department of Urology and Organ Transplantation, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China;Institution of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China;Department of Hematology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China;Department of Urology and Organ Transplantation, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China;Department of Urology and Organ Transplantation, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China;Department of Urology and Organ Transplantation, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China |
| Abstract: | |
| Keywords: | Buyang Huanwu Decoction brain death (BD) inflammatory cytokine p38 mitogen-activated protein kinase (p38 MAPK) |
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