喉鳞癌中P糖蛋白表达与细胞增殖、凋亡及预后的关系

目的 探讨P糖蛋白在喉鳞癌组织中表达与肿瘤细胞的增殖、凋亡活性，及其临床病理特征与患者预后的关系。方法 应用免疫组化技术检测86例患者喉鳞癌组织中P糖蛋白、Ki 67的表达，TUNEL法测定喉鳞癌细胞凋亡率。结果 P糖蛋白在喉鳞癌组织中的阳性表达率为39.5％(34/86)，与喉鳞癌的临床分期、组织学分级、淋巴结转移及患者术后生存率有关(均P＜0.05)；P糖蛋白表达阳性组的肿瘤细胞凋亡率比阴性组低(P＜0.05)，而增殖率差异无统计学意义(P＞0.05)。结论 P糖蛋白可能对喉鳞癌细胞的凋亡活性具有一定抑制作用；P糖蛋白阳性表达率对于评估喉鳞癌病程进展及预后具有一定价值。

Significance of apoptotic and proliferative indices and P-glycoprotein expression in laryngeal squamous cell carcinoma

Objective To explore the relationship of P-glycoprotein expression with the apoptotic index, proliferative index, clinicopathological characteristics, and their prognostic values in laryngeal squamous cell carcinoma (LSCC). Methods Samples from 86 patients with LSCC were analyzed for P-glycoprotein and Ki 67 expression with immunohistochemistry, and the apoptotic index with the TUNEL method. The association of P-glycoprotein expression with clinicopathological parameters, apoptotic and proliferative activities, and patients′ overall survival was subsequently analyzed. Results It was found that up-regulation of P-glycoprotein expression was associated with decreased apoptosis (P＜0.05) but not with the changes in proliferation (P＞0.05). Expression of P-glycoprotein was significantly related to the clinical stage, histological grading, lymph node metastasis and overall survival in LSCC (P＜0.05). Conclusion These results show that the overexpression of P-glycoprotein may abrogate the apoptosis of LSCC cells. Furthermore, the results demonstrate that P-glycoprotein is predictive of malignant progression and prognosis of LSCC.