| RP-HPLC法测定木香大安颗粒中橙皮苷的含量 |
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| 引用本文： | 韩飞,熊魏,罗哓健.RP-HPLC法测定木香大安颗粒中橙皮苷的含量[J].齐鲁药事,2011,30(12):694-695,702. |
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| 作者姓名： | 韩飞 熊魏 罗哓健 |
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| 作者单位： | 1. 江西中医学院,江西南昌,330004
2. 中药固体制剂制造技术国家工程研究中心,江西南昌,330006 |
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| 基金项目： | 江西省卫生厅中医药科研基金 |
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| 摘 要： | 目的建立高效液相色谱法测定木香大安颗粒中橙皮苷的含量.方法采用反相高效液相色谱法,C18色谱柱,流动相为乙腈-6.0%醋酸溶液(79∶21),检测波长283 nm,流速1.0 mL爛min-1,柱温30℃.结果橙皮苷进样量在0.036 3～2.321 9 ug(r=0.999 9)范围内线性关系良好,样品平均回收率为98.89%,RSD为1.10%.结论本方法简便、准确、分离度好、重现性高,可作为木香大安颗粒的质量控制方法.
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| 关 键 词： | 木香大安颗粒 橙皮苷 含量测定 高效液相色谱法 |
Determination of hesperidin in Muxiang Daan Granules by RP - HPLC |
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| HAN Fei,XIONG Wei,LUO Xiao-jian.Determination of hesperidin in Muxiang Daan Granules by RP - HPLC[J].qilu pharmaceutical affairs,2011,30(12):694-695,702. |
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| Authors: | HAN Fei XIONG Wei LUO Xiao-jian |
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| Institution: | 1. Jiangxi University of Traditional Chinese Medicine, Nanehang 330004, China ;2. Chinese Medicine Solid Preparations National Engineering Research Center for Manufacturing Technology, Nanchang 330006, China ) |
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| Abstract: | Objective To establish an HPLC method for the determination of hesperidin in Muxiang Daan Granules. Methods RP - HPLC was adopted with C18 column,the mobile phrase was acetonitrile - 6.0% acetic acid （ 79：21 ） , the detection wavelength was 283 nm, the flow rate was 1.0 mL min - 1 and the column temperature was 30 ℃. Results The sample of hesperidin showed a good linear relationship within the range of 0.036 3× 2. 321 9 μg （ r = 0. 999 9 ） . The average recovery was 98. 89% and the RSD was 1.10%. Conclusion The method was simple, accurate, of high resolution and good reproducibility, and it can be used for the quality control of Muxiang Daan Granules. |
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| Keywords: | Muxiang Daan Granules Hesperidin Determination HPLC |
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