Characteristics of three human gastric cancer cell lines,NU-GC-2, NU-GC-3 and NU-GC-4 |
| |
Authors: | Seiji Akiyama MD Hiroyuki Amo Tadashi Watanabe Mutsushi Matsuyama Junichi Sakamoto Munehisa Imaizumi Hidehito Ichihashi Tatsuhei Kondo Hiroshi Takagi |
| |
Affiliation: | (1) Department of Surgery, Nagoya National Hospital, 4-1-1, Sannomaru, Naka-ku, 460 Nagoya, Japan;(2) Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute, Nagoya, Japan;(3) Second Department of Surgery, School of Medicine, Nagoya University, Nagoya, Japan;(4) Department of Thoracic Surgery, School of Medicine, Nagoya University, Nagoya, Japan |
| |
Abstract: | Three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4 were establishedin vitro from the cancer tissues obtained from 3 patients during surgery. The pathological findings of the gastric tumors of these cases revealed poorly differentiated adenocarcinoma (and partial signet-ring cell carcinoma in the case of NU-GC-4). NU-GC-2 and NU-GC-4 were originally obtained from metastatic paragastric lymph nodes and NU-GC-3 was obtained from a metastatic tumor in the brachial muscle. The cells of NU-GC-2 and NU-GC-3 are polygonal in shape and grow as a monolayer sheet. NU-GC-4 cells, however, are mainly spherical in shape with a few free floating cells. Electron microscopy revealed epithelial characteristics in all 3 cell lines. The average doubling time of NU-GC-2 was 36.1 hours, that of NU-GC-3 was 38.2 hours and that of NU-GC-4 was 29.9 hours. The modal chromosome number of NU-GC-2 was 62, that of NU-GC-3 was 58 and those of NU-GC-4 grown inin vitro andin vivo were 52–54 and 53, respectively.In vitro andin vivo lines of NU-GC-4 were established from the same tumor. These two cell lines are quite similar in morphology, but slightly different in karyotype. Thein vitro sensitivity to anticancer agents was highest in NU-GC-4 and lowest in NU-GC-2. Of the anticancer agents, mitomycin C and adriamycin were most effective on the cells of all 3 cell lines. |
| |
Keywords: | gastric carcinoma in vitro cell lines in vivo cell lines gastric cancer cell lines |
本文献已被 SpringerLink 等数据库收录! |
|