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| 快速蛋白液相色谱系统纯化肺癌组织热休克蛋白70及其鉴定 | |
| 引用本文： | 张慧珍,杨继要,范清堂,许东,吴逸明.快速蛋白液相色谱系统纯化肺癌组织热休克蛋白70及其鉴定[J].郑州大学学报(医学版),2007,42(1):20-22. |
| 作者姓名： | 张慧珍 杨继要 范清堂 许东 吴逸明 |
| 作者单位： | 1. 郑州大学公共卫生学院环境卫生学教研室,郑州,450001 2. 郑州大学基础医学院组织学与胚胎学教研室,郑州,450001 3. 郑州大学公共卫生学院劳动卫生学教研室,郑州,450001 |
| 基金项目： | 国家自然科学基金 , 河南省自然科学基金 , 河南省郑州市科技攻关项目 |
| 摘 要： | 目的:从肺癌组织中纯化并鉴定热休克蛋白70(HSP70),为研究其免疫原性和作为肿瘤疫苗奠定基础.方法:用组织蛋白裂解液从肺癌组织中提取总蛋白,应用ConA-sepharose亲和层析和快速蛋白液相色谱系统(FRLC)连续分离、梯度洗脱获得蛋白,应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳及Western-blot进行蛋白相对分子质量及免疫原性鉴定.结果与结论:所获蛋白的相对分子质量为70 000,经Western-blot鉴定为HSP70. |
| 关 键 词： | 快速蛋白液相色谱系统 肺癌组织 热休克蛋白70 |
| 收稿时间： | 2006-03-14 |
| 修稿时间： | 2006-03-14 |
Purification and identification of heat shock protein 70 from lung cancer tissue using fast. protein liquid chromatography system |
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| ZHANG Huizhen,YANG Jiyao,FAN Qingtang,XU Dong,WU Yiming.Purification and identification of heat shock protein 70 from lung cancer tissue using fast. protein liquid chromatography system[J].Journal of Zhengzhou University: Med Sci,2007,42(1):20-22. | |
| Authors: | ZHANG Huizhen YANG Jiyao FAN Qingtang XU Dong WU Yiming |
| Institution: | 1 Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001 2. Department of Embryology and Histology, College of Basic Medical Sciences, Zhengzhou University,Zhengzhou 450001 3 |
| Abstract: | Aim: To separate and purify heat shock protein 70(HSP70) from lung cancer tissue in order to study its immunogenecity and make it a new type tumor vaccines.Methods:The whole soluble proteins of lung cancer tissue were extracted and separated by ConA-sepharose affinity column chromatography and fast protein liquid chromatography system. The relative molecular weight and the immunogenecity of the obtained protein were confirmed by SDS-PAGE and Western-blot.Results and Conclusion: The obtained protein was confirmed to be HSP70 with relative molecular weight of 70 000. |
| Keywords: | fast protein liquid chromatography system lung cancer tissue HSF70 |
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