老年胃癌及癌前病变中p16及cyclinD1蛋白的表达

目的　探讨老年胃癌及癌前病变中p16、cyclinD1蛋白的表达、相互关系及其意义。　 方法 　应用免疫组化链霉卵白素 (SP)法检测老年胃癌、不典型增生、萎缩性胃炎、浅表性胃炎及正常胃组织中p16、cyclinD1蛋白的表达。　结果　p16蛋白在正常胃粘膜表达率最高 (85 7% ) ,并随病变进展 (浅表性胃炎 -萎缩性胃炎 -不典型增生 -腺癌 ) ,表达率呈下降趋势 (分别为 84 4% ,76 9% ,2 9 0 % ,3 4 3 % ) ,其中不典型增生 ,腺癌组与正常组比较有显著差异 (P <0 0 5 ) ,cy clinD1蛋白随病变发展 (萎缩性胃炎、不典型增生、腺癌 )表达率升高 (分别为 19 2 % ,3 8 7% ,5 5 7% ) ,其中腺癌与萎缩性胃炎比较差异显著 (P <0 0 5 )。相关性分析显示大多数受检老年胃癌组织中 ,p16与cyclinD1呈反向表达。　 结论　p16和cyclinD1在胃上皮癌变过程中起着重要作用 ,其在胃癌中的反向表达趋势提示两者可能存在相互抑制机制。p16蛋白低表达和cyclinD1蛋白高表达可能是胃癌发生过程中的早期分子事件。

Significance of p16,cyclinD1 expression in elderly patients with gastric carcinoma and precancerous lesion

Objective To evaluate the significance of p16,cyclinD1 protein expression and the relationships between them with gastric carcinoma and precancerous lesion in elderly patients. Methods SP immunohistochemical method was used to detect the expression of p16,cyclinD1 in gastric carcinoma,atypical hyperplasia,atrophic gastritis,superficial gastritis and normal gastric mucosa. Results The positive expression rate of p16 was the highest in normal gastric mucosa and decreased with the progression from superficial gastritis to atrophic gastritis to atypical hyperplasia and to adenocarcionma(84 4%,76 9%,29 0%,34 3%,respectively).The positive expression rates of cyclinD1 increased with the lesions progressing from atrophic gastritis to atypical hyperplasia and to adenocarcionoma(19 2%,38 7%,55 7% respectively).There was a significant difference between adenocarcinoma group and atrophic gastritis group( P <0 05).The inverse expression between p16 and cyclinD1 was shown in most of the tested gastric cancer. Conclusions p16 and cyclinD1 play important roles in gastric carcinogenesis,and the low expression of p16 and overexpression of cyclinD1 may be an early molecular event in gastric carcinogenesis.