苯乙酸钠诱导Jurkat细胞凋亡机制研究

目的:探讨苯乙酸钠(Sodium pheny-acetate,NaPA)诱导Jurkat(人粒细胞白血病细胞株)细胞凋亡机制及对细胞周期各时相的影响。方法:应用MTT比色法及流式细胞仪检测NaPA对Jurkat细胞的增值抑制率及细胞周期各时相的变化,并通过电子显微镜观察Jurkat细胞的亚细胞结构改变。结果:NaPA对Jurkat细胞增殖有明显的抑制作用,抑制率为27%~58%,且呈剂量依赖性。流式细胞仪结果表明,G0/G1期细胞增多,S期细胞减少,G2/M期细胞相对增多,细胞凋亡率增高,电子显微镜下可见线粒体肿胀,细胞核固缩。结论:NaPA能够抑制Jurkat细胞G1期向S期转化进程,诱导Jurkat细胞凋亡。

Mechanism of Jurkat cell apoptosis induced by Sodium pheny-acetate

Objective To investigate the mechanism of Jurkat cell(human myelocytic leukaemia) apoptosis and cell cycle alteration induced by Sodium pheny-acetate(NaPA).Method MTT and FACS were applied to detect Jurkat cell proliferation inhibition rate and cell cycle alteration treated by NaPA.Electron microscope was used to observe subcellular structure alteration of Jurkat cell.Results NaPA could inhibit Jurkat cell proliferation rate which varied from 27%~58% with dose dependent.FACS analysis showed cell in stage G0/G1 increased and in stage S decreased with the relative increase of stage G2/M,Jurkat cell apoptosis rate increased.Mitochondria swelling and karyopycrosis could be observed under the electron microscope.Conclusion NaPA can inhibit Jurkat cell cycle progression from stage G1 to S and induced Jurkat cell apoptosis.