Ca^2＋对人精子穿透无透明带金黄地鼠卵的影响

应用人精子穿透无透明带金黄地鼠卵（SPA）技术，在BWW培养液中分别以不同浓度的Ca^ （1．71mmol,3.42mmol,5.13mmol,1.71mmol和ETA0．50mmol,1.71mmol和EGTA1．71mmol),对10例人精液标本进行分析，研究Ca^ 对人精子体外获能和穿透无透明带金黄地鼠卵的生物学效应，同时用透射电镜观察精子的亚细胞结构变化。结果表明：对照组（Ca^ 1．71mmol)穿透率为82．2％，而试验组的穿透率显著降低（P＜0．01），分别为9．5％，0，17．2％，0。Ca^ 对人精子亚微结构的影响主要发生在精子的顶体和颈部。本试验初步表明Ca^ 与人精子获能和顶体反应存在着一定的依存关系，Ca^ 浓度过高（＞1．71mmol）或过低（＜1．71mmol)，对精子获和穿透能力都有明显的抑制作用。

INFLUENCE OF CALCIUM IONS ON HUMAN SPERM PENETRATING INTO ZONE-FREE HAMSTER OVA

The human/hamster system was used in this study to evaluate the influences of various concentrations of Calcium ions ( Ca2+ ) in BWW ( Big-gers-Whitten-Wh:ttingham ) medium on the human sperm capacitating in vitro and penetrating into zona-free hamster ova. The results of these experiments ( specimens from 10 donors ) showed that the control group (Ca2+ 1.71mmol ) penetrating rate was 82.2%, and that the rates of the test groups ( Ca2+3.42, 5.13mmol, Ca2+1.71+ EGTA 0.5mmol and Ca2+1.71+EGTA 1.71mmol ) were decreased significantly ( p<0.01 ), being 9.5%, 0, 1.72 %, and O respectively. These findings indicate that the relations were presented between calcium ions and capacitation and acrosome reaction of the sperm. Calcium ions at higher ( 1.71mmol ) or lower ( 1.71mmol ) concentrations may have an inhibitory effect on sperm penetration. Therefore there may be great latent value of Ca2+ and its chelators EGTA in the development of contraceptives.