基质金属蛋白酶及抑制物在胎膜早破中表达

目的 探讨基质金属蛋白酶-9、-2及其抑制因子-1、-2在胎膜早破孕妇的胎膜中的变化及与胎膜早破的关系.方法 应用免疫组织化学S-P法对上述两组胎膜组织检测基质金属蛋白酶-9、基质金属蛋白酶抑制因子-1、基质金属蛋白酶-2、基质金属蛋白酶抑制因子-2的表达.结果 胎膜早破组和对照组胎膜中基质金属蛋白酶-9、基质金属蛋白酶-2的阳性表达分别为100%、87%,差异有显著性(P＜0.01);胎膜早破组胎膜中基质金属蛋白酶抑制因子-1、基质金属蛋白酶抑制因子-2的表达较正常孕妇组低,但差异无显著性(P＞0.05).结论 基质金属蛋白酶-9、基质金属蛋白酶-2在胎膜早破孕妇胎膜中高表达,可能是胎膜早破发生的原因之一,需及时采取预防措施,防止胎膜早破的发生;人工诱导基质金属蛋白酶抑制因子-1、基质金属蛋白酶抑制因子-2或降低基质金属蛋白酶-2、基质金属蛋白酶-9的含量以防止胎膜早破的发生、发展,有望成为胎膜早破预防及治疗的辅助手段.

Expression of matrix metalloproteinases in premature rupture of fetai membrane

Objective To explore the changes of matrix metalloproteinase(MMP-9,MMP-2)and tissue inhibitors of matrix metalloproteinase(TIMP-1,TIMP-2)in fetal membranes of pregnant women with premature rupture of membranes(PROM),and their relationships with PROM.Methods The expression of MMP-9,TIMP-1,MMP-2,TIMP-2 in fetal membranes was detected by immunohistochemistry S-Pmethod.Results The positive expression of MMP-9 and MMP-2 in fetal membranes in PROM group and control group was 100% and 87%,respectively,there were significant differences(P<0.01).The TIMP-1 and TIMP-2 expression in the fetal membranes of PROM groups were lower than that in normal pregnant women group.But there was no difference(P>0.05).Conclusion The MMP-9,MMP-2 is highly expressed in fetal membranes,which may be one of causes of PROM,and preventive measures should be taken to prevent PROM.Artificially induced expression of TIMP-1,TIMP-2 or decreasing the content of MMP-9,MMP-2 probably inhibits occurrence and development pathogenesis of PROM.This study may provide a new auxiliary way for treatment and prevention of PROM.