RIG-I激活抑制肠道病毒71型的复制

目的探讨细胞内病毒识别受体RIG-I激活后对EV71复制的影响。方法分别用Poly(I:C)和表达RIG-I N端的质粒转染RD细胞,24 h后感染EV71,感染24 h后收获细胞和上清。提取细胞RNA并反转录为cDNA,Real-time PCR测定细胞中RIG-I与IFN-β的mRNA的表达水平和EV71的RNA水平。用免疫印迹的方法检测RIG-I和EV71的蛋白表达水平。将感染病毒的细胞上清进行病毒滴度的测定。结果 RIG-I被激活之后RIG-I与IFN-β的mRNA的表达水平上升,并且RIG-I的蛋白表达可以被检测到。感染细胞中的EV71的RNA水平和病毒蛋白表达水平下降,并且病毒滴度显著降低。结论 RIG-I的激活以及其诱导的IFN-β对EV71的复制有抑制效应。

RIG-I activation inhibits enterovirus 71 replication

Activation of innate immune pathogen sensor retinoic acid-inducible gene I (RIG-I) has recently been shown to induce antiviral state. In this study, we aim to study the effect of the intracellular viral sensor RIG-I activation on EV71. RD cells were transfected with Poly (I:C) or plasmid expressing RIG-I N-terminal. At 24 h after transfection, cells were infected with EV71; at 24 h post-infection, cells were harvested for RNA and protein analysis and cell culture supernatants were collected for determinatio...