| 两株PRRSV分离株ORF5与Nsp2基因部分序列分析 |
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| 引用本文： | 祝秀梅,吕志慧,牟克斌.两株PRRSV分离株ORF5与Nsp2基因部分序列分析[J].免疫学杂志,2011(7):562-566,571. |
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| 作者姓名： | 祝秀梅 吕志慧 牟克斌 |
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| 作者单位： | 中农威特生物科技股份有限公司; |
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| 摘 要： | 目的分析沈阳和甘肃发病猪场的2株猪繁殖与呼吸综合征病毒(PRRSV)的ORF5基因和Nsp2基因变异情况。方法采用RT-PCR方法,对ORF5基因序列和Nsp2基因部分序列进行扩增、克隆并测序。并用DNAStar软件将测序结果与国内外发表的10株参考毒株进行比对分析。结果 2株分离株ORF5基因与国内外其它美洲型分离株核苷酸的同源性为88.6%～98.7%,推导的氨基酸的同源性为86.6%～98.0%;Nsp2基因的核苷酸同源性为73.4%～99.8%,推导的氨基酸的同源性为68.6%～99.5%,且该基因与国内其他变异株有完全一致的缺失特征。结论这两株分离株均属于PRRSV美洲型变异株,为该病的防治及疫苗的设计奠定理论基础。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF5基因 Nsp2基因 遗传变异 |
Sequence analysis for ORF5 gene and partial Nsp2 gene of two PRRSV strains |
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| ZHU Xiumei,L Zhihui,MU Kebin.Sequence analysis for ORF5 gene and partial Nsp2 gene of two PRRSV strains[J].Immunological Journal,2011(7):562-566,571. |
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| Authors: | ZHU Xiumei L Zhihui MU Kebin |
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| Institution: | China Agricultural Veterinary Biological Science and Technology Co.Ltd.,Lanzhou,730046,China |
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| Abstract: | This study aimed to analyze the genetic variation of the ORF5 and Nsp2 gene in two PRRSV isolates from ShenYang and GanSu province.The ORF5 sequence and partial Nsp2 sequence were amplified by RT-PCR and the PCR products were cloned and sequenced.Multiple sequences of ORF5 and Nsp2 gene nucleotides as well as amino acids were compared with those of 10 reported PRRSV strains and analyzed using DNA Star software.The results showed that homology between ORF5 gene of two PRRSV isolates and other American genoty... |
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| Keywords: | Porcine reproductive and respiration syndrome virus ORF5 gene Nsp2 gene Genetic variation |
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