精氨酸对大肠癌患者免疫功能及肿瘤细胞增殖活性的影响

目的探讨精氨酸(Arg)对大肠癌患者免疫功能及肿瘤细胞增殖活性的影响. 方法大肠癌患者34例,随机分2组.实验组静脉输注Arg 25 g/d、连续3 d.检测细胞免疫指标T细胞亚群,NK细胞活性,淋巴细胞转化率;红细胞免疫指标C3b受体花环率(C3bERR),免疫复合物花环率(ICR), 循环免疫复合物(CIC)以及体液免疫指标.免疫组织化学方法和流式细胞仪研究肿瘤细胞增殖活性的变化. 结果实验组CD4+、CD4+/CD8+、NK细胞活性、C3bRR均有提高,尤以CD4+、NK细胞活性提高明显(P<0.01);淋巴细胞转化率和CIC亦有改善,但仅组内比较差异显著(P<0.05); C3bRR变化与NK细胞活性变化之间呈正相关(r=0.5804,P<0.05);增殖细胞核抗原(PCNA)表达显著下降,由55.8%降至42.6%,(P<0.01),S期%及(S+G2+M)期%亦显著降低(P<0.05),而对照组各指标均无显著变化(P>0.05). 结论短期应用药理剂量的Arg可以改善大肠癌患者的细胞免疫、红细胞免疫指标,抑制肿瘤细胞的增殖活性.

The effect of L-arginine on immune function and tumor proliferating activity in patients with colorectal cancer

Objective In order to study the effect of L-arginine on the immune function and tumor proliferating activity in patients with colorectal cancer. Methods 34 patients with colorectal cancer were randomly divided into the experimental group, in which patients received hydrochloride arginine 25gram/day for three days, and the control group. The changes of peripheral blood cellular immune parameters such as T lymphocyte subsets, lymphocyte transformation rate(LTR) and natural killer(NK) cell activity, red blood cell(RBC) immune parameters as C 3b RR, ICR and circulating immune compound(CIC) were determined, and at the same time,humoral immune parameters, tumor PCNA and FCM were also studied. Results In the experimental group, there were significant improvement on CD 4 ,CD 4 /CD 8 , NK cell activity, RBC-C 3b RR, especially CD 4 and NK cell activity( P < 0.01 ). LTR and CIC increased only compared with that of pre-arginine supplementation. RBC-ICR decreased without statistical significence( P > 0.05 ). There was positive correlation between the change of C 3b RR and that of NK cell activity( r= 0.5804 ,P < 0.05 ). PCNA expression of tumour was inhibited from 55.8% to 42.6% significantly( P < 0.01 ). The percentage of S phase and (S G 2 M) phase in the cancer decreased( P < 0.05 ). Conclusions The short term application of L-arginine in pharmaceatical dosage could improve cellular immunity and RBC immunity and inhibit tumor proliferating activity.