| RP-HPLC法测定毛冬青缓释片中原儿茶醛的含量 |
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| 引用本文： | 李苑新,袁旭江,朱盛山.RP-HPLC法测定毛冬青缓释片中原儿茶醛的含量[J].中药新药与临床药理,2005,16(4):267-269. |
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| 作者姓名： | 李苑新 袁旭江 朱盛山 |
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| 作者单位： | 广东药学院中药开发研究所,广州,510224 |
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| 摘 要： | 目的建立测定毛冬青缓释片中原儿茶醛含量的方法.方法采用反相高效液相色谱法,色谱柱:AgilentC8柱(4.6×150 mm,5 μm),流动相:甲醇-1%冰乙酸水溶液(梯度洗脱),柱温:30℃,检测波长:312 nm,流速:1 mL/min.结果在11.7～140.4 ng范围内,原儿茶醛与色谱峰面积呈良好的线性关系,r=0.99995,平均加样回收率为96.41%,RSD=1.04%(n=5).结论该方法准确,稳定性、重现性好,适于毛冬青缓释片中原儿茶醛的含量测定,可有效地控制产品质量.
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| 关 键 词： | 毛冬青缓释片 原儿茶醛 反相高效液相色谱法 含量测定 |
| 文章编号： | 1003-9783(2005)04-0267-03 |
| 修稿时间： | 2005年3月22日 |
Determination of the Protocatechualdehyde in Maodongqing Sustained - release Tablet by RP - HPLC |
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| LI Yuanxin,YUAN Xujiang,ZHU Shenshang.Determination of the Protocatechualdehyde in Maodongqing Sustained - release Tablet by RP - HPLC[J].Traditional Chinese Drug Research & Clinical Pharmacology,2005,16(4):267-269. |
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| Authors: | LI Yuanxin YUAN Xujiang ZHU Shenshang |
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| Abstract: | Object To establish a new method to determine protocatechualdehyde in Maodongqing Sustained- release Tablet. Methods The RP- HPLC method was applied with CH3OH and 1 % acetic acid as mobile phase, flowrate at 1.0 mL/min, and detection wavelength at 312 nm. Results In the range from 11.7 ng to 140.4 ng, protocatechualdehyde was in a good line with peak area, r=0.99995, the average recovery ratio was 96.41 % , RSD=1.04 % (n=5). Conclusions It was the first time to establish the RP- HPLC method to determine and control the protocatechualdehyde in Maodongqing Sustained- release Tablet with accuracy and a good reproducibility. |
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| Keywords: | Maodongqing Sustained- release Tablet Protocatechualdehyde RP- HPLC Content determination |
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