少突胶质细胞肿瘤染色体1p、19q和10q杂合性缺失与临床预后的关系

目的 探讨少突胶质细胞肿瘤微卫星变异的遗传和分子特性及其与临床预后的关系。方法 26例少突胶质细胞瘤和25例伴有少突胶质细胞瘤成分的胶质母细胞瘤成对的血和肿瘤标本DNA提取后，进行微卫星不稳定性分析染色体1、19q和10q杂合性缺失。结果 54％少突胶质细胞瘤检出1pLOH，58％检出19qLOH，35％检出10q LOH，其中50％间变性少突胶质细胞瘤出现10q LOH。1p／19q LOH相伴存，二者密切相关（P〈0．0001）；40％GBMO检出1pLOH，仅4例1p LOH伴10q LOH；60％检出19q LOH，64％检出10q LOH。结论 1P和19q LOH是少突胶质细胞瘤分子和遗传特性之一，并且与化疗敏感和预后好有关，10qLOH是少突胶质细胞瘤进展标志。1PLOH与长PFS有关，10qLOH与短PFS有关。GBMO分子表型不同于GBM。

Relationship between loss of heterozygosity on chromosome 1p, 19q and 10q and clinical prognosis in oligodendroglial tumors

Purpose To identify the characteristics of molecular profile and clinical prognosis by analysis of microsatellite alterations in oligodendroglial tumors. Methods DNA of blood and tumor tissue were extracted from 26 cases of pure oligodendrogliomas and 25 glioblastomas with an oligodendroglial component (GBMO). DNA was screened to loss of heterozygosity (LOH) on chromosome 1,19q and 10q using microsatellite polymorphic markers. Results Loss of heterozygosity(LOH)on chromosome 1p, 19q and 10q were detected by 54%, 58% and 35% in 26 oligodendrogliomas respectively. 10q LOH was found in 50% of anaplastic oligodendrogliomas. 1p and 19q LOH were closely correlated (P<0.000 1). 40 % of GBMO appeared 1p LOH, and 4 GBMOs appeared both 1p LOH and 10q LOH. 60% of samples were detected 19q LOH and 64% of samples presented 10q LOH. Conclusions Oligodendrogliomas are characterized by frequent combined LOH on chromosome 1p and 19q. LOH on 10q may correspond to anaplastic oligodendrogliomas and GBMO. The value of LOH 1p as a predictor of longer PFS, and LOH on 10q is a significant predictor of shorter PFS. The molecular profile of GBMO is distinct from GBM.