米非司酮和孕酮对子宫内膜异位症患者子宫内膜白细胞介素6分泌的影响

目的探讨米非司酮和孕酮对子宫内膜异位症(内异症)患者异位子宫内膜(异位内膜)与正常子宫内膜(在位内膜)白细胞介素6(IL-6)分泌的影响.方法采用酶联免疫吸附试验(ELISA)方法,检测米非司酮浓度为1×10-6mol/L(米非司酮1组)、1×10-4mol/L(米非司酮2组),和孕酮浓度为1×10-7mol/L(孕酮1组)、1×10-5mol/L(孕酮2组)与体外培养的异位内膜细胞和在位内膜细胞上清液作用后的IL-6水平.结果米非司酮可降低异位内膜细胞IL-6水平,米非司酮1组和米非司酮2组的IL-6分为(1914.33±799.28)μg/L和(990.25±58.40)μg/L,两组与空白组比较(下同),差异有极显著性(P＜0.01).孕酮也可降低异位内膜细胞IL-6水平,孕酮1组和孕酮2组的IL-6分为(2575.89±119.75)μg/L和(1736.25±750.89)μg/L(P＜0.01).米非司酮还可使在位内膜细胞IL-6水平降低,其中米非司酮1组和米非司酮2组分别为(346.96±24.32)μg/L和(270.22±36.15)μg/L(P＜0.01).孕酮对在位内膜细胞IL-6水平无明显影响(P＞0.05).结论米非司酮和孕酮可抑制异位内膜和在位内膜细胞IL-6的分泌.

Regulatory effects of mifepristone and progesterone on the secretion of interleukin-6 by cultured eutopic and etopic endometrial cells

OBJECTIVE: To investigate the regulatory effects of mifepristone and progesterone on the secretion of interleukin-6 (IL-6) by endometrial and endometriosis cells in vitro. METHODS: Primary cultures of eutopic and ectopic endometrial cells from 9 cases of endometriosis were exposed to mifepristone (10(-6) mol/L, 10(-4) mol/L) and progesterone (1 x 10(-7) mol/L, 1 x 10(-5) mol/L) respectively. IL-6 secretion was analyzed in the culture medium by enzyme linked immunosorbent assay (ELSA). RESULTS: Mifepristone inhibited the IL-6 secretion of ectopic endometrial cells, with the concentrations of IL-6 was (1 914.33 +/- 799.28) microg/L in the 1 x 10(-6) mol/L group (P < 0.01) and (990.25 +/- 58.40) microg/L in the 10(-4) mol/L group (P < 0.01). Progesterone also inhibited IL-6 secretion of ectopic endometrial cells, with (2 575.89 +/- 119.75) microg/L in the 1 x 10(-7) mol/L group (P < 0.05) and (1 736.25 +/- 750.89) microg/L in the 10(-5) mol/L group (P < 0.01). Mifepristone restrained the secretion of IL-6 by eutopic endometrial cells, with (346.96 < or = 24.32) microg/L in the 1 x 10(-6) mol/L group (P < 0.01) and (270.22 +/- 36.15) microg/L in the 1 x 10(-4) mol/L group (P < 0.01). All the effects of down regulation were more obvious when the concentrations of mifepristone and progesterone increased (P < 0.05 - 0.01) . Secretion of IL-6 by eutopic endometrial cells did not change significantly when incubated with progesterone (P > 0.05). CONCLUSION: The inhibitory effects on the secretion of IL-6 by ectopic and (or) eutopic endometrium may provide one of the cellular therapeutic mechanisms of mifepristone and progesterone on endometriosis.