Peripheral blood expansion of early progenitor cells after high-dose cyclophosphamide and rhGM-CSF |
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| Institution: | 1. Dipartimento di Medicina ed Oncologia Sperimentale, Cattedra di Ematologia-Ospedale Molinette, Università di Torino, Via Genova 3, 10126 Torino, Italy;2. Divisione di Oncologia Medica, Unità di Trapianto di Midollo Osseo “Cristina Gandini”, Istituto Nazionale Tumori di Milano, Milano, Italy;3. Istituto di Scienze Mediche, Università di Milano, Milano, Italy;1. Department of Radiology, National Cancer Center, Goyang, Republic of Korea;2. Department of Surgery, National Cancer Center, Goyang, Republic of Korea;3. Center for Liver Cancer, National Cancer Center, Goyang, Republic of Korea;4. Department of Radiology, Seoul National University Hospital, Seoul, Republic of Korea.;1. Department of Surgery, Asahikawa Medical University;2. Department of Mechanical Engineering, Tokyo Metropolitan University;3. Department of Pathology, Asahikawa Medical University;4. Department of Pediatrics, Asahikawa Medical University;5. Department of Chemistry, Nara Medical University;1. Department of Neurosurgery, University of Colorado School of Medicine, Denver, Colorado;2. Division of Nephrology, Department of Medicine, Rutgers-Robert Wood Johnson Medical School, New Brunswick, New Jersey;4. Division of Cardiology, Department of Medicine, Rutgers-Robert Wood Johnson Medical School, New Brunswick, New Jersey;3. Department of Surgery, Rutgers-Robert Wood Johnson Medical School, New Brunswick, New Jersey;5. Kidney/Pancreas Transplant Program, Robert Wood Johnson University Hospital, New Brunswick, New Jersey;2. Department of Surgery, Northwell Health, Sandra Atlas Bass Center for Liver Diseases, Manhasset, New York;1. National Centre for Cell Science, Ganeshkhind, Pune 411 007, India;2. Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721102, India |
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| Abstract: | In 20 patients with non-Hodgkin lymphoma or breast cancer, high-dose cyclophosphamide induced, during the post-nadir period of rapid leucocyte recovery, on median day 19 about a 30-fold increase in the peak concentration of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E) colony-forming cells, and an even higher increase in the more immature pluripotent progenitors (CFU-Mix, 72-fold). After infusion of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), peak concentration was reached earlier (median day 15) and with further enhancements (159, 116 and 283-fold, respectively, in the number of CFU-GM, BFU-E and CFU-Mix). Most CFU-GM were immature, lacking the differentiation antigen CD15, and gave rise to large myeloid colonies, reflecting a high proliferative capacity of the founder cells. Very immature maphosphamide-resistant progenitors were detectable. The marked expansion in the circulating pool was predictable and reliable, allowing harvesting, after two or three leukaphereses, of sufficient haematopoietic progenitors for autologous bone-marrow reconstitution. |
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