Growth factor requirements of human colorectal tumour cells: Relations to cellular differentiation |
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| Affiliation: | 1. Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, Hawaii;2. Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California;1. Department of Oncologic Pathology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts;8. Department of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts;3. Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;6. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;12. Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;1010. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;4. Department of Epidemiology and Biostatistics, Ministry of Education Key Lab of Environment and Health, School of Public Health, Huazhong University of Science and Technology, Wuhan, People''s Republic of China;5. Channing Division of Network Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts;12123. Program in MPE Molecular Pathological Epidemiology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts;1212. Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts;7. The 7th Affiliated Hospital of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, People''s Republic of China;10. Clinical and Translational Epidemiology Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts;9. Division of Gastroenterology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts;71. Medical Oncology Department 2, Chinese People''s Liberation Army General Hospital, Beijing, People''s Republic of China;123. Cancer Center, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People''s Republic of China;84. College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, People''s Republic of China;106. Oncology Department, The First Affiliated Hospital of Chinese People''s Liberation Army General Hospital, Beijing, People''s Republic of China;95. Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, Massachusetts;77. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts;88. Broad Institute of Massachusetts Institute of Technology, Harvard, Cambridge, Massachusetts;99. Yale Cancer Center, New Haven, Connecticut;771. Smilow Cancer Hospital, New Haven, Connecticut;1. Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA;2. Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA;3. Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA;1. Department of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts;2. Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;3. Department of Epidemiology and Biostatistics, and the Ministry of Education Key Lab of Environment and Health, School of Public Health, Huazhong University of Science and Technology, Wuhan, People’s Republic of China;4. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;5. Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;6. Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts;7. Channing Division of Network Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts;8. Clinical and Translational Epidemiology Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts;9. Division of Gastroenterology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts;10. Medical Oncology Department 2, Chinese People''s Liberation Army General Hospital, Beijing, People’s Republic of China;11. College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, People’s Republic of China;12. Division of Pathology, The Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan;13. Department of Gastroenterology and Hepatology, Sapporo Medical University School of Medicine, Sapporo, Japan;14. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts;15. Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Boston, Massachusetts;16. Yale Cancer Center, New Haven, Connecticut;17. Department of Medicine, Yale School of Medicine, New Haven, Connecticut;18. Smilow Cancer Hospital, New Haven, Connecticut;19. Department of Oncologic Pathology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts;1. Department of Microbiology & Immunology, Stanford University School of Medicine, 299 Campus Drive, Stanford, CA 94305-5124, USA;2. Department of Medicine, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5107, USA;3. Infectious Diseases Section, Veterans Affairs Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, CA 94304-1207, USA;1. Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 61265 Brno, Czech Republic;2. Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic;3. Institute of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Sokolská 581, 500 05 Hradec Králové, Czech Republic |
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| Abstract: | Human colorectal tumour lines that exhibit different degrees of differentiation were studied to define their growth requirements. The poorly differentiated cell lines SW620, SW480, SW48 and SW837 proliferated in Dulbecco's modified Eagle's medium without exogenously added growth factors. In contrast, the moderately differentiated cell lines SW1222, HT29, PC/JW and LS174T proliferated only in medium supplemented with growth factor. SW1222 and HT29 cells required transferrin for growth, which was improved by other growth-promoting factors including epidermal growth factor (SW1222) and sodium selenite (HT29). PC/JW and LS174T required both insulin and transferrin for optimal growth. The tumour cell lines could be passaged continuously in serum-free medium supplemented with growth factor and in some cases they grew better than in serum-supplemented medium. The serum-free growth conditions should prove useful for studies on differentiation in colorectal cell lines and their interactions with growth factors. |
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