Tissue plasminogen activator and placental plasminogen activator inhibitor in human gingival fluid |
| |
| Institution: | 1. Laboratory of Precision Oncology, China Medial University School of Pharmacy, Shenyang, Liaoning, China;2. Department of Surgical Oncology and Breast Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China;3. Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China;4. Department of Radiation Oncology, China Japan Friendship Hospital, Beijing, China;5. Department of Breast Surgery, Liaoning Cancer Hospital, Shenyang, Liaoning, China;6. Department of Otolaryngology, University of Colorado School of Medicine, Aurora, CO 80045, USA;1. iTHES Research Group and Theoretical Molecular Science Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;2. Laboratory for Biomolecular Function Simulation, RIKEN Quantitative Biology Center, Integrated Innovation Building 7F, 6-7-1 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan;3. Faculty of Biology-Oriented Science and Technology, KINDAI University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan;4. Department of Molecular Sciences and Center for Computational Biology, The University of Kansas, 2030 Becker Drive, Lawrence, KS 66047, United States;5. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, United States;6. Department of Chemistry, Michigan State University, East Lansing, MI 48824, United States;7. Computational Biophysics Research Team, RIKEN Advanced Institute for Computational Science, 7-1-26 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan;1. German Breast Group, Neu-Isenburg;2. Oncological Practice Bethanien, Cancer Center Frankfurt Northeast, Frankfurt am Main;3. HELIOS Klinikum Berlin-Buch, Berlin;4. Brustzentrum, Universitätsfrauenklinik Ulm, Ulm;5. Institute of Pathology, Charité-Universitätsmedizin Berlin, Berlin;6. Berlin Institute of Health (BIH), Berlin;7. Gynäkologie mit Brustzentrum, Charité-Universitätsmedizin Berlin, Berlin;8. Universitätsfrauenklinik Tübingen, Tübingen;9. Rotkreuzklinikum München Frauenklinik, München;10. Zentrum für Pathologie, Zytologie und Molekularpathologie Neuss, Neuss;11. Medical Center, Luisenkrankenhaus Düsseldorf, Düsseldorf;12. Brustzentrum, Sana-Klinikum Offenbach, Offenbach;13. Praxisklinik für Hämatologie und Onkologie Koblenz, Koblenz;14. Breast Unit, Kliniken Essen-Mitte, Essen;15. Klinik für Frauenheilkunde, Universitätsklinikum Freiburg, Freiburg;16. Brustzentrum, Universitätsklinikum Erlangen, Erlangen;17. Center for Hereditary Breast and Ovarian Cancer, University Hospital Cologne, Cologne;18. National Center for Tumor Diseases, University Hospital Heidelberg, Heidelberg, Germany |
| |
| Abstract: | Gingival crevicular fluid (GCF) is an extracellular exudate protecting periodontal tissue. Pathological changes in the periodontium are reflected in the composition of GCF. Crevicular fluid was collected from healthy volunteers on Millipore® filter disks, and eluted at 100-fold dilution. The samples were tested for fibrinolytic activity and the presence of the plasminogen activators, t-PA and u-PA, and the specific plasminogen activator inhibitors, PAI-1 and PAI-2. In the diluted samples, t-PA was found at concentrations of 4–33 gmg/l, and PAI-2 at concentrations of 19–84 μg/l, whereas u-PA and PAI-1 were hardly detectable. Analyses of parotid and whole saliva yielded no evidence of gingival fluid contamination from these sources. The fibrinolytic activity of gingival fluid was completely quenched both by antibodies against t-PA and by PAI-2, indicating the presence of t-PA in its two chain form which is more susceptible to inhibition. This inhibition by PAI-2 may serve a regulatory purpose and prevent excessive proteolysis and tissue destruction. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
