新型双相钙磷陶瓷复合骨形态发生蛋白及碱性成纤维细胞生长因子修复兔骨缺损

摘要背景：细胞因子能启动、促进并维持软骨和骨生成，但需要合适的载体才能发挥其生物学活性。目的：验证复合骨形态发生蛋白及碱性成纤维细胞生长因子的新型双相钙磷陶瓷(1∶1)促进骨缺损修复的作用。方法：27只兔，造成兔两侧股骨中下段4 mm×4 mm×12 mm的缺损,随机分为3组：骨形态发生蛋白+ 碱性成纤维细胞生长因子+双相钙磷陶瓷组、骨形态发生蛋白+双相钙磷陶瓷组、双相钙磷陶瓷组。于4，8，12周取材，通过苏木精-伊红染色，X射线以及计算机图像分析，比较各组缺损的修复情况。结果与结论：骨形态发生蛋白+碱性成纤维细胞生长因子+双相钙磷陶瓷组及骨形态发生蛋白+双相钙磷陶瓷组在软骨诱导、小梁骨的形成数量、骨缺损修复等方面均明显优于双相钙磷陶瓷组。骨形态发生蛋白+碱性成纤维细胞生长因子+双相钙磷陶瓷组又优于骨形态发生蛋白+双相钙磷陶瓷组，在8周时编织骨小梁逐渐改建为成熟的板层骨及髓腔结构，骨缺损基本修复。12周时完全修复。结果提示：①双相钙磷陶瓷复合骨形态发生蛋白及碱性成纤维细胞生长因子在体内有较强的成骨活性，可以促进骨缺损的修复。②碱性成纤维细胞生长因子与骨形态发生蛋白合用可以加快新骨的形成。③新型双相钙磷陶瓷是一个吸附细胞因子的良好缓释载体，它的降解吸收和新骨的形成是一个相互促进过程。关键词：双相钙磷陶瓷；骨缺损；诱导成骨；骨形态发生蛋白；成纤维细胞因子doi:10.3969/j.issn.1673-8225.2010.42.001

Hydroxyapatite/tricalcium phosphate combined with bone morphogenetic protein and basic fibroblast growth factor repairs bone defects in rabbits

AbstractBACKGROUND: Cytokines can initialize, promote and maintain cartilage and bone formation, but require a suitable carrier to exert biological activity.OBJECTIVE: To investigate the repaired effects of bone defected by the compound of hydroxyapatite/tricalcium phosphate (1:1, HA/TCP) combined with bone morphogenetic protein (BMP) and basic fibroblast growth factor (bFGF).METHODS: A 4 mm × 4 mm × 12 mm bone defect was produced in both thighbone of 27 experimental rabbits, which were randomly divided into three groups, BMP + bFGF + HA/TCP, BMP + HA/TCP, HA/TCP groups. The repair of bone defects in each group was investigated by means of hematoxylin-eosin staining, X-ray examination, and computer image analysis at 4, 8, 12 weeks after operation.RESULTS AND CONCLUSION: BMP + bFGF + HA/TCP group and BMP + HA/TCP group were superior to HA/TCP group regarding the cartilage induction, trabecular bone formation and bone defects repair. In addition, BMP + bFGF + HA/TCP group was better than BMP + HA/TCP group. At 8 weeks after operation, woven bone trabecular gradually developed into mature lamellar bone and pulp cavity, bone defects were almost repaired. At 12 weeks, bone defects were completely repaired. Results indicated that the compound of HA/TCP, BMP and bFGF possesses strong osteogenesis potential and the ability of rebuilding bone defect. BMP combined with bFGF can apparently promote new bone formation. The porous HA/TCP is a good controlled-release scaffold material and can absorb cytokines, its degradation and absorption are interacted with new bone formation.