| 高压喷射载VEGF165、bFGF凝胶促缺血心肌血管新生的实验研究 |
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| 引用本文： | 熊鹿,黄晶,周大燕,郭睿,吴奇,周志益.高压喷射载VEGF165、bFGF凝胶促缺血心肌血管新生的实验研究[J].解放军医学杂志,2010,35(5). |
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| 作者姓名： | 熊鹿 黄晶 周大燕 郭睿 吴奇 周志益 |
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| 作者单位： | 重庆医科大学附属第二医院心内科,重庆,400010 |
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| 基金项目： | 国家自然科学基金重点项目(30527001);;国家自然科学基金面上项目(3067087);;重庆市科技攻关计划项目(CSTS2009AB5003) |
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| 摘 要： | 目的探讨高压喷射心肌内给药的可行性以及载VEGF165、bFGF温敏型壳聚糖水凝胶对缺血心肌的促血管新生和心肌保护作用。方法健康杂种犬32只,随机均分为单纯心肌梗死(SMI)组,高压喷射生理盐水(NS)组,高压喷射温敏型壳聚糖水凝胶(Chitosan)组,高压喷射载VEGF165、bFGF温敏型壳聚糖水凝胶(Chitosan+VEGF165+bFGF)组。结扎左冠状动脉前降支制备急性心肌梗死模型后,SMI组直接关胸,NS组、Chitosan组和Chitosan+VEGF165+bFGF组在结扎线以下左室前壁行高压喷射心肌内给药8～10次,1次/cm2。术后6周取材行组织学、免疫组化检查并计算心肌梗死面积,处死动物前行血流动力学检测。结果术后6周心肌内仅有少量壳聚糖水凝胶样物质残留,其周围无明显的炎性细胞浸润;与SMI组比较,Chitosan组和Chitosan+VEGF165+bF-GF组梗死区新生血管数量增加(P<0.05),梗死面积缩小(P<0.05),左室舒张末压和左室内压最大下降速率明显改善(P<0.05)。结论高压喷射心肌内给药安全、可行;载VEGF165、bFGF温敏型壳聚糖水凝胶心肌内注射能促进...
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| 关 键 词： | 注射 喷射 壳聚糖 水凝胶 血管内皮生长因子类 成纤维细胞生长因子2 新生血管化 病理性 |
An experimental study on the effect of injection of VEGF165-and bFGF-incorporated gel under high pressure on promoting angiogenesis in ischemic myocardium |
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| XIONG Lu,HUANG Jing,ZHOU Da-yan,GUO Rui,WU Qi,ZHOU Zhi-yi.An experimental study on the effect of injection of VEGF165-and bFGF-incorporated gel under high pressure on promoting angiogenesis in ischemic myocardium[J].Medical Journal of Chinese People's Liberation Army,2010,35(5). |
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| Authors: | XIONG Lu HUANG Jing ZHOU Da-yan GUO Rui WU Qi ZHOU Zhi-yi |
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| Abstract: | Objective To explore the feasibility of high-pressure injection of VEGF165-and bFGF-incorporated thermo-sensitive chitosan hydrogel into ischemic myocardium, and to observe the effects of VEGF165-and bFGF-incorporated chitosan hydrogel on promoting angiogenesis and protection of myocardium from ischemia. Methods Thirty two dogs were randomly divided into 4 groups (8 each): simple myocardial infarction (SMI) group, injection of normal saline (NS) under high pressure group, injection of chitosan hydrogel unde... |
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| Keywords: | injections jet chitosan hydrogel vascular endothelial growth factors fibroblast growth factor 2 neovascu larization pathologic |
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