High-resolution fluorescencein situ hybridization ofRBM- andTSPY-related cosmids on released Y chromatin in humans and pygmy chimpanzees |
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| Authors: | Christian Conrad Thomas Hierl Birgitta Gläser Kay Taylor Sylvia Zeitler Ann C. Chandley Werner Schempp |
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| Affiliation: | (1) Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106 Freiburg, Germany;(2) Galton Laboratory, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE London, UK;(3) Present address: MRC Human Genetics Unit, Western General Hospital, EH4 2XU Edinburgh, UK |
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| Abstract: | Applying two-colour fluorescencein situ hybridization (FISH) we simultaneously hybridizedRBM- andTSPY-related cosmids to Y chromosomes in prophase and to released Y chromatin in interphase nuclei of man and pygmy chimpanzee. Whereas, even on prophasic Y chromosomes, no resolution of the overlappingRBM andTSPY signal clusters could be achieved, theRBM andTSPY signals are completely separated from each other in our maximum released Y chromatin stretches in interphase nuclei. These results unequivocally lend support to the view that theRBM andTSPY families have an interspersed organization on the Y chromosomes of man and higher apes. Thus, the distribution ofRBM andTSPY signals might well go back to a common organization of these genes next to each other on an ancient Y chromosome.accepted for publication by M. Schmid |
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| Keywords: | high-resolution fluorescence in situ hybridization spermatogenesis genes Y chromosome evolution |
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