| 内脂素促进巨噬细胞向致血栓表型转化 |
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| 引用本文： | 张高峰,许澎,乔棋. 内脂素促进巨噬细胞向致血栓表型转化[J]. 中国分子心脏病学杂志, 2010, 10(6) |
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| 作者姓名： | 张高峰 许澎 乔棋 |
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| 作者单位： | 复旦大学附属上海市第五人民医院心内科,上海市,200240 |
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| 摘 要： | 目的研究内脂素对巨噬细胞中组织因子(TF)和纤溶酶原激活物抑制剂-1(PAI-1)表达的影响及可能的机制。方法体外培养的THP-1细胞中加入不同浓度的内脂素(包括空白对照,50ng/ml,100ng/ml三个浓度组),每组分别孵育6小时,12小时和24小时各3个时间点。同时设立2-羟基萘甲基磷酸三乙酸甲基酯(HNMPA;100umol/L)组。将各组细胞进行realtime PCR和Western blot检测,比较各组TF和PAI-1表达的情况。结果内脂素增加巨噬细胞中TF和PAI-1的mRNA转录和蛋白质表达水平,HNMPA不影响内脂素的这些效应。结论内脂素促进巨噬细胞向促血栓表型转化,机制与胰岛素受体相关的途径无关。
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| 关 键 词： | 内脂素 巨噬细胞 血栓形成 动脉粥样硬化 |
Visfatin Promoted Macrophages Conversion to a Thrombogenic Phenotype Independent of Insulin Receptor Signalling |
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| ZHANG Gao-feng,XU Peng,QIAO Qi. Visfatin Promoted Macrophages Conversion to a Thrombogenic Phenotype Independent of Insulin Receptor Signalling[J]. Molecular Cardiology of China, 2010, 10(6) |
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| Authors: | ZHANG Gao-feng XU Peng QIAO Qi |
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| Affiliation: | ZHANG Gao-feng; XU Peng; QIAO Qi.The fifth People s Hospital of Shanghai; Fudan University; Shanghai 200240; China; |
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| Abstract: | Objective To investigate effects of visfatin on expression of TF and PAI-1 in macrophages and related mechanism.Methods We treated THP-1-derived macrophages with visfatin ranged from 0 to100ng/ml for 6 to 24 hours,and tested mRNA expression and protein production of TF and PAI-1 by realtime quantified-PCR and Western blot analysis respectively.By administering HNMPA-an insulin receptor antagonist,we intended to determine whether visfatin influence TF and PAI-1 expressions through insulin receptor signalling... |
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| Keywords: | Visfatin Macrophage Thrombosis Atherosclerosis |
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