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The origin of unknown source DNA from touched objects
Affiliation:1. Deakin University, School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology, (Waurn Ponds Campus), 75 Pigdons Road, Locked Bag 20000, Geelong, Victoria 3217, Australia;2. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, Macleod, Victoria 3085, Australia;1. Division Biological Traces, Netherlands Forensic Institute, P.O. Box 24044, 2490 AA, The Hague, The Netherlands;2. Swedish National Forensic Centre, SE-581 94, Linköping, Sweden;3. Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83, Linköping, Sweden;4. Forensic Science Ireland, Garda HQ, Phoenix Park, Dublin 8, Ireland;5. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 94248, 1090 GE Amsterdam, The Netherlands;6. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod, Vic. 3085, Australia;7. School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology, Deakin University, Locked Bag 20000, Geelong, Vic. 3220, Australia;1. School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia;2. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, Macleod, Victoria, Australia;3. School of Psychology and Public Health, La Trobe University, Bundoora, Australia;1. School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology, Deakin University, Waurn Ponds, Australia;2. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, Macleod, Macleod, Australia;3. School of Molecular Sciences, College of Science, Health and Engineering, La Trobe University, Bundoora, Australia;1. School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology, Deakin University, (Waurn Ponds Campus), Geelong, Victoria, Australia;2. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, Macleod, Victoria, Australia;3. School of Psychology and Public Health, La Trobe University, Bundoora, Victoria, Australia
Abstract:The presence of DNA in a criminal investigation often requires scrutiny in relation to how it came to be where it was found. There is a paucity of data with respect to the extent to which one can assume that the last person handling an object, which has previously been touched by others, will contribute to the DNA profile generated from it. There are limited data in detailing the extent to which any foreign DNA is picked-up from a previously touched object and transferred to subsequently touched objects. This study focuses on DNA transfer and persistence on a knife handle after multiple handlings with the knife by different individuals soon after each other, as well as handprints left on flat DNA-free surfaces immediately after touching a knife handle with a known history of prior handling.The profiles of later handlers of a knife are more prominent than earlier handlers; however, the last handler is not always the major contributor to the profile. Proportional contributions to the profiles retrieved from knife handles vary depending on the individuals touching the knife handle. They can also vary when knife handles have been handled in the same manner by the same individuals in the same sequence on different occasions. Hands readily pickup DNA left on objects by others and transfer it to subsequently touched objects. The quantity of foreign DNA picked up by a hand and deposited on subsequently touched objects diminishes as more DNA-free objects are handled soon after each other.Caution is advised when considering how DNA from different individuals may have been transferred to the object from which it was collected.
Keywords:DNA transfer  Trace DNA  Touch DNA  Non-self DNA  Knife handle
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