膜型基质金属蛋白酶-1在黏着斑激酶相关非激酶调控肝星状细胞胶原代谢中的作用

目的： 探讨黏着斑激酶相关非激酶（FRNK）对纤维连接蛋白（FN）刺激的肝星状细胞（HSC）膜型基质金属蛋白酶-1（MT1-MMP）表达的影响并探讨MT1-MMP在FRNK调控HSC胶原代谢中的作用。方法：应用体外细胞培养技术，脂质体介导法进行FRNK质粒瞬时转染；采用Western blotting法测定FRNK蛋白在瞬时转染时相应的表达，鉴定转染效果；分别应用Western blotting和RT-PCR方法测定MT1-MMP在HSC中的相应表达。结果：FRNK质粒成功转染HSC，于转染48 h时，FRNK蛋白表达最强，P<0.05；FRNK转染后在基因水平上：MT1-MMP mRNA表达明显增加；翻译蛋白水平上：FRNK质粒转染HSC 48 h后，MT1-MMP蛋白表达量显著升高。结论：脂质体介导下FRNK质粒转染，可使外源性FRNK在HSC内大量表达，FRNK可能通过上调MT1-MMP值来抑制HSC胶原合成。

Effects of MT1-MMP on collagen metabolism regulated by FRNK in hepatic stellate cells

AIM: To investigate the effect of FAK-related non-kinase (FRNK) on the expression of membrane-type matrix metalloproteinase-1 (MT1-MMP) in hepatic stellate cells (HSC). METHODS: FRNK were transfected into HSCs by cationic liposome method. The protein levels of FRNK in HSC were assayed by Western blotting. The levels of MT1-MMP were determined by RT-PCR for mRNA and by Western blotting for protein, respectively. RESULTS: The up-regulated expression of FRNK protein was observed and it was at 48 h after transfection that the FRNK protein content was the highest (P<0.05). The expressions of MT1-MMP mRNA and protein were also up-regulated by the transfection of FRNK, and it was at 48 h after transfection that the MT1-MMP protein content was significantly increased. CONCLUSION: The mRNA and protein of FRNK were over-expressed in HSC transfected with the gene of FRNK. The inhibitory effect of FRNK on the collagen synthesis in HSC may be through the up-regulation of MT1-MMP.