| 苦参素诱导卵巢癌HO8910细胞凋亡及细胞周期的影响 |
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| 引用本文： | 王英,高洪泉,杨春壮. 苦参素诱导卵巢癌HO8910细胞凋亡及细胞周期的影响[J]. 牡丹江医学院学报, 2004, 25(1): 4-7 |
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| 作者姓名： | 王英 高洪泉 杨春壮 |
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| 作者单位： | 牡丹江医学院,157011;牡丹江医学院,157011;牡丹江医学院,157011 |
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| 摘 要： | 目的：探讨苦参素对卵巢癌细胞株H08910的抑制增殖效应及凋亡诱导作用。方法：用苦参素处理H08910细胞，采用MTT法检测药物对细胞的抑制作用，倒置显微镜观察细胞形态学改变；琼脂糖凝胶电泳观察DNA降解，以及应用流式细胞仪观察细胞凋亡过程中细胞周期的变化。结果：在苦参素作用下，H08910细胞呈凋亡改变，DNA琼脂糖凝胶电泳呈典型的凋亡特征。细胞凋亡的同时，细胞周期发生特定的改变。结论：苦参素能抑制卵巢癌细胞增殖，能诱导卵巢癌细胞凋亡。
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| 关 键 词： | 苦参素 细胞凋亡 卵巢癌细胞株 |
| 修稿时间： | 2003-12-01 |
OXYMATRINE INDUCES APOPTOSIS AND EFFECT OF CELL CYCLE IN OVARIAN CANCER CELL LINES HO8910 |
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| Wangying et al. Mudanjiang Medical College. OXYMATRINE INDUCES APOPTOSIS AND EFFECT OF CELL CYCLE IN OVARIAN CANCER CELL LINES HO8910[J]. Journal of Mudanjiang Medical College, 2004, 25(1): 4-7 |
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| Authors: | Wangying et al. Mudanjiang Medical College |
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| Affiliation: | Wangying et al. Mudanjiang Medical College 157011 |
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| Abstract: | The current study was designed to investigate whether Oxymatrine can induces apoptosis in Ovarian Cancer cell. Methods: Cell proliferation was assessed by MTT assays. Morphological changes of apoptosis were observed with invert microscope . DNA ladder and cell cycle were examined by DNA agarose gel eletronphores and PI fluorescence flow cytometry(FCM) ,respectively. Results:Under the influence of Oxymatrine, H08910 cell exhibited changes of apoptosis both in morphology and in the characteristics of electrophoresis. Meanwhile the cell cycle also showed special change. Conclusion: Oxymatrine can inhibit cell proliferation and induces apoptosis in Ovarian Cancer cell. |
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| Keywords: | Oxymatrine apoptosis ovarian cancer cell line |
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