血管内皮生长因子-C对鼻咽癌CNE-2细胞小鼠移植瘤的辐射敏感性的影响

  目的  探讨干扰VEGF-C表达可以增加鼻咽癌CNE-2细胞小鼠移植瘤辐射敏感性作用以及相关信号途径。  方法  鼻咽癌CNE-2小鼠移植瘤随机分为:瘤体组；siRNA-NC组；siRNA-VEGF-C组；照射组；siRNA-NC+照射组；siRNA-VEGF-C+照射组。观察肿瘤生长情况；采用免疫组化检测VEGF-C、ATM、P65蛋白的表达变化。  结果  干扰VEGF-C表达鼻咽癌CNE-2细胞小鼠移植瘤，在照射后明显肿瘤生长明显变慢。免疫组化检测结果显示:干扰VEGF-C小鼠移植瘤后，VEGF-C、ATM明显减少（P < 0.05），P65增加（P < 0.05）；联合照射后，VEGF-C、ATM明显减少（P < 0.05）。  结论  在鼻咽癌CNE-2细胞小鼠移植瘤中，干扰VEGF-C基因通过激活NF-kB信号通路影响下游ATM，进而增加放疗敏感性。

Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice

  Objective   To investigate the effect of interfering with the expression of VEGF-C on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cell transplanted tumor and related signaling pathways.   Methods  The mice with transplanted tumors of nasopharyngeal carcinoma CNE-2 were randomly divided into tumor body group, siRNA-NC group, siRNA-VEGF-C group; irradiation group; siRNA-NC+ irradiation group; siRNA-VEGF-C+irradiation group. Tumor growth was observed, and the protein expression of VEGF-C, ATM and P65 were detected by immunohistochemistry.   Results  Interference of VEGF-C expression in nasopharyngeal carcinoma CNE-2 cell transplanted tumors showed significantly slower tumor growth after irradiation. Immunohistochemical test results showed that after tumor transplantation of mice interfered with VEGF-C, VEGF-C and ATM were significantly decreased （P < 0.05）, while P65 was increased （P < 0.05）. After combined irradiation, VEGF-C and ATM were significantly decreased （P < 0.05）.  Conclusion  In mouse nasopharyngeal carcinoma CNE-2 cell transplantation tumors, interfering with VEGF-C gene affects downstream ATM by activating NF-kB signaling pathway, thereby increasing the sensitivity of radiotherapy.