基于Oncomine数据库研究PDE4D基因在卵巢癌中的表达及血根碱的调控作用

目的 通过深度挖掘Oncomine数据库中PDE4D在卵巢癌的研究数据,分析PDE4D基因在卵巢癌中的表达变化,并进行细胞实验研究血根碱对卵巢癌细胞中PDE4D基因表达的影响.方法 PharmMapper数据库查询与血根碱药效基团匹配的作用靶点,收集Oncomine数据库PDE4D在卵巢癌中的研究数据,进一步分析PDE...

Expression of PDE4D Gene in Ovarian Cancer Based on Oncomine and the Regulation of Sanguinarine

  Objective   To investigate the expression and clinical significance of PDE4D in ovarian cancer by excavating gene information in Oncomine and explore the effect of sanguinarine on PDE4D by cells experiment.   Methods   PharmMapper was used to find the targets matched with the pharmacodynamic groups of sanguinarine. The gene information about PDE4D in ovarian cancer research was collected in Oncomine, and the expression level was analyzed. The A2780 and SKOV3 ovarian carcinoma cells were divided into control group and sanguinarine group. CCK8 and RT-qPCR were used to detect the effects of sanguinarine on the proliferation and PDE4D mRNA expression of A2780 and SKOV3 cells.   Results   PDE4D was the target matched with the pharmacodynamic group of sanguinarine. There were 6 studies referred to PDE4D in ovarian cancer and normal sample collected in the Oncomine. The expression of PDE4D in tumor tissues was significantly higher than that in normal （P < 0.05）. The cell experiments showed that compared with the control group, the proliferation of A2780 and SKOV3 cells was significantly inhibited and the expression of PDE4D mRNA was decreased in the sanguinarine group, with statistical significance （P < 0.05）.   Conclusions   PDE4D gene may play a role in the occurrence and development of ovarian cancer, and sanguinarine can inhibit the growth of ovarian cancer cells, which may be related to the down-regulation of PDE4D expression.