DEAD盒RNA解螺旋酶5和微小RNA-205在前列腺癌中的表达及临床意义

目的 检测DEAD盒RNA解螺旋酶5（DDX5）和微小RNA-205（miR-205）在前列腺癌（PCa）中的表达情况，并分析二者与PCa预后的关系。方法 选取2015年4月至2017年4月于北京市朝阳区双桥医院行前列腺癌根治术或穿刺活检术的86例PCa患者为研究对象，取患者术中切除的癌组织及癌旁组织分别作为PCa组和对照组。采用实时荧光定量PCR（qRT-PCR）法检测组织中DDX5 mRNA、miR-205的水平。采用免疫组化法检测组织中DDX5的表达情况。分析DDX5、miR-205表达水平与临床病理特征的关系。采用Kaplan-Meier生存分析DDX5、miR-205表达水平与PCa患者的预后关系。采用Cox回归分析PCa预后不良的影响因素。结果 PCa组的DDX5 mRNA及蛋白表达水平均高于对照组（均P<0.05），miR-205表达水平低于对照组（P<0.05）。PCa患者中DDX5、miR-205表达与肿瘤分期、血清PSA、Gleason评分、肿瘤转移均有相关性（均P<0.05），而与患者年龄、切缘性质均无相关性（均P>0.05）。经Kaplan-Meier生存分析，DDX5阳性表达患者的3年累积生存率低于DDX5阴性表达患者（P<0.05）；miR-205高表达患者的3年累积生存率高于miR-205低表达患者（P<0.05）。多因素Cox分析结果显示，DDX5水平偏高、miR-205水平偏低是PCa不良预后的危险因素（HR=2.598、3.342，均P<0.01）。结论 在PCa患者癌组织中DDX5高表达，miR-205低表达，二者与PCa的多种临床病理及预后有关，是PCa患者预后不良的危险因素。

Expression and clinical significance of DEAD box helicase 5 and microRNA-205 in prostate cancer

Objective To detect the expression of DEAD box helicase 5 (DDX5) and microRNA-205 (miR-205) in prostate cancer (PCa), and to analyze their relationship with the prognosis of PCa.Methods A total of 86 patients with PCa who underwent radical prostatectomy or needle biopsy in shuangqiao hospital of chaoyang district from April 2015 to April 2017 were selected as the research objects. The cancer tissues and adjacent tissues were taken as PCa group and control group respectively. Real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the levels of DDX5 mRNA and miR-205 in the tissues of the subjects. Immunohistochemical method was used to detect the expression of DDX5. The relationship between the expression of DDX5, miR-205 and clinicopathological features was analyzed. Kaplan-Meier survival analysis was used to analyze the relationship between the expression levels of DDX5 and miR-205 and the prognosis of patients with PCa. Cox regression analysis was used to analyze the influencing factors of poor prognosis of PCa.Results The expression levels of DDX5 mRNA and protein in PCa group were higher than those in control group (all P<0.05), and the expression levels of miR-205 were lower than those in control group (P<0.05). The expressions of DDX5 and miR-205 in PCa patients were correlated with tumor stage, serum PSA, Gleason score and tumor metastasis (all P<0.05), but not with age and nature of resection margin (all P>0.05). Kaplan-meier survival analysis showed that the 3 year cumulative survival rate of DDX5 positive patients was lower than that of DDX5 negative patients (P<0.05). The 3 year cumulative survival rate of patients with high miR-205 expression was higher than that of patients with low miR 205 expression (P<0.01). Multivariate Cox analysis showed that higher DDX5 level and lower miR-205 level were risk factors for poor prognosis of PCa (HR=2.598, 3.342, all P<0.01).Conclusions DDX5 was highly expressed in the cancer tissues of PCa patients, while miR-205 was low expressed, which were associated with various clinicopathological and prognosis of PCa, and were risk factors for poor prognosis of PCa patients.