吲哚菁绿荧光腹腔镜技术在困难胆囊切除中的临床应用

目的 研究吲哚菁绿荧光染色在腹腔镜胆囊切除术中的安全性、可行性及可靠性，能否有效避免胆道损伤，探讨吲哚菁绿荧光腹腔镜技术在困难胆囊切除中的不足与优势。方法 回顾性分析，收集昆明医科大学第二附属医院自2017年12月1日至2019年12月1日因胆囊结石并反复胆囊炎发作行胆囊切除术65例，男性27例，女性38例，年龄29～67岁，符合困难胆囊切除术入组标准的患者临床资料。65例患者随机分组，其中常规腹腔镜手术组35例（对照组，男性14例，女性21例），吲哚菁绿荧光腹腔镜组30例（实验组，男性13例，女性17例）。分析比较2组患者性别、年龄、基础疾病等一般资料，以及手术时间、术中出血量、术后排气时间、术后住院天数、住院总费用、术前及术后肝功（ALT、AST、血清白蛋白、总胆红素）、肾功（肌酐）、能否有效识别胆管解剖结构以及识别胆道时间。结果 对照组手术时间较实验组长（P <0.05）；在分离寻找胆囊三角解剖结构的时间上，对照组时间长于实验组时间（P <0.05）；对照组术中出血量与实验组无统计学意义（P> 0.05）；对照组术后通气时间、住院总费用、住院时间与实验组无明显差...

Clinical Application of Laparoscopic Cholecystectomy with Fluorescence Cholangiography of Indocyanine Green in the Difficult Cholecystectomy

  Objective  To study the safety, feasibility and reliability of indocyanine green fluorescence staining in laparoscopic cholecystectomy, whether it can effectively avoid bile duct injury, and to explore the shortcomings and advantages of indocyanine green fluorescence laparoscopic technology in difficult cholecystectomy.   Methods  The method of retrospective analysis was adopted, and the clinical data of patients meeting inclusion criteria for difficult cholecystectomy in 65 cases of cholecystectomies with the causes of the gallstone and recurrent cholecystitis in The Second Affiliated Hospital of Kunming Medical University from December 1, 2018 to December 1, 2019 were collected. All patients had signed the informed consent and had no history of iodine allergy. 65 patients were assigned randomly with 35 cases of laparoscopic cholecystectomy （LC） and 30 cases of laparoscopic cholecystectomy （LC） with fluorescence cholangiography of indocyanine green （ICG）. All kinds of the preoperative, intraoperative and postoperative data were collected to compare between the conventional group and cholangiography group. The application effect, safety and feasibility of the laparoscopic technologies with fluorescence cholangiography in the difficult cholecystectomy were explored, including whether the anatomic structure of the bile duct and the biliary tract time can be identified effectively.   Results  The time of laparoscopic cholecystectomy （LC） group was significantly longer than that of fluorescence cholangiography group （P < 0.05）; in terms of the time to isolate the anatomy of biliary tract, the time of fluorescence cholangiography group was significantly shorter than that of laparoscopic cholecystectomy （LC） group （P < 0.05）; the amount of bleeding in laparoscopic cholecystectomy （LC） was no statistically significant combined with fluorescence cholangiography group （P > 0.05）; there was no significant difference in length of stay （LOS） and all-in cost of stay between the two groups （P > 0.005）.   Conclusions  Indocyanine green fluorescence imaging laparoscopy has high operability and strong safety in identifying the anatomical structure of biliary tract in difficult cholecystectomy. It can effectively identify the anatomical structure of gallbladder triangle and effectively avoid the injury of biliary tract and blood vessels; Indocyanine green fluorescence imaging laparoscopic technology has the advantage of short operation time compared with traditional laparoscopic technology in Difficult Cholecystectomy, and there is no significant difference in postoperative liver function, infection index, hospitalization cycle and total hospitalization cost.