不同3D打印精度制作的生物材料表面形貌对表皮葡萄球菌生物膜形成影响

目的 探讨不同3D打印精度制作的生物材料表面形貌对表皮葡萄球菌生物膜形成的影响.方法 以医用3D打印原材料光敏树脂MED610为材料,使用光固化成型技术分别按照16 μm、30μm、100 μm层厚制作样本.测量样本表面粗糙轮廓的算术平均偏差Ra、轮廓的最大高度Rz,静态角接触法检测样本疏水性.与表皮葡萄球菌标准株RP...

Effect of Surface Topography on Staphylococcus Epidermidis Biofilm Formation by Different 3D Printing Thickness of Biomaterials

  Objective  To investigate the effect of surface topography on staphylococcus epidermidis biofilm formation by different 3D printing Thickness of biomaterials.   Methods  Medical 3D printing raw material, photosensitive resin MED610 was used as the material and samples were made according to 16 μm, 30 μm and 100 μm layer thicknesses using light-curing molding technology, respectively. Measure the sample surface roughness contour arithmetic mean deviation Ra, contour maximum height Rz, static angular contact method to detect sample hydrophobicity. The samples were co-cultured with Staphylococcus epidermidis standard strain RP62A on an oscillator, and the material surface was removed at 2 h, 6 h, 12 h, 24 h and 30 h, respectively. The number of bacterial communities per field of view was measured by laser confocal microscopy and the biofilm formation on the material surface was observed by scanning electron microscopy.   Results  The Ra and Rz values on the surface of the 16-μm layer thickness fabricated biomaterial were smaller than those of the 30-μm layer thickness and 60-μm fabricated samples, and there was no significant difference in the hydrophobicity of the surface of the fabricated materials with different layer thicknesses. When co-cultured with Staphylococcus epidermidis for 2 h and 6 h, a small amount of Staphylococcus epidermidis was scattered on the surface of the material in the 16 μm group without bacterial aggregation, and the number of bacterial communities per unit field of view was significantly lower than that in the 30 μm and 100 μm groups （P < 0.05）. Biofilm formation could be observed on the material surface of each group at 12 h, 24 h and 30 h of incubation and there was no significant difference in the number of bacterial communities per unit field of view （P > 0.05）.  Conclusions  Different precision fabrication has a large effect on the surface roughness of 3D printing materials, but has no significant effect on hydrophobicity. The 16-μm layer thickness fabrication material is less favorable for Staphylococcus epidermidis adhesion in the early stage of infection than the 30-μm and 100-μm layer thickness fabrication materials.