| AK4对肝内胆管癌细胞HUCCT1增殖、迁移的影响 |
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| 作者姓名： | 廖周俊 杨少华 刘立鑫 胡晟 陈轶晖 康强 张小文 |
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| 作者单位： | 昆明医科大学第二附属医院肝胆胰外科，云南 昆明 650101 |
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| 基金项目： | 国家自然科学基金资助项目（81760430）；云南省万人计划“名医”培养基金资助项目[（云人社12018）P73号] |
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| 摘 要： | 目的 探究AK4对肝内胆管癌细胞HUCCT1增殖、迁移能力的影响。 方法 采用小干扰RNA（siRNA）技术靶向沉默肝内胆管癌细胞HUCCT1中AK4的表达，采用免疫印迹法检测沉默效果以及筛选siRNA-AK4，通过EdU实验和细胞划痕实验检测该胆管癌细胞增殖、迁移能力。 结果 通过免疫印迹法检测出siRNA-AK4-3沉默效果最好，EdU实验显示沉默AK4后，细胞增殖能力下降（P < 0.05），细胞划痕实验显示沉默AK4后，细胞迁移能力下降（P < 0.01）。 结论 沉默肝内胆管癌细胞HUCCT1中AK4的表达后，其增殖、迁移能力受到抑制。
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| 关 键 词： | 肝内胆管癌 AK4 增殖 迁移 |
| 收稿时间： | 2022-03-26 |
Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1 |
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| Institution: | Dept. of Hepatobiliary Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China |
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| Abstract: | Objective To investigate the effects of AK4 on the proliferation and migration of intrahepatic bile duct carcinoma cells HUCCT1. Methods Small interfering RNA （siRNA） technology was used to target the silencing of AK4 expression in HUCCT1 of intrahepatic bile duct cancer cells. Western blot was used to detect the transfection efficiency of siRNA and screen siRNA-AK4. The proliferation and migration ability of the bile duct cancer cells were detected by EdU assay and cell scar assay. Results EdU experiment showed that cell proliferation decreased after AK4 silencing （P < 0.05）, the cell migration ability was decreased after AK4 silencing （P < 0.01）. Conclusion AK4 can enhance the proliferation and migration of HUCCT1 in intrahepatic bile duct carcinoma cells. |
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