miR-133b 靶向 PTBP1 对肾细胞癌增殖和侵袭的影响

目的 探讨 miR-133b 靶向多聚嘧啶区结合蛋白1 (polyrimidine tract binding protein 1, PTBP1) 对肾细胞癌 (renal cell carcinoma, RCC) 增殖和侵袭的影响。 方法 检测 miR-133b 在 RCC 癌组织及细胞中的表达水平; 验证 miR-133b 与 PTBP1 的靶向关系及 miR-133b 表达对肾癌细胞 PTBP1 表达及增殖、 迁移的影响。 结果 与癌旁组织比较, miR-133b 在 RCC 组织中表达下降, 而 PTBP1 上升 (P< 0. 05)。 PTBP1 是miR-133b 的靶基因; 与 miR-NC 组比较, miR-133b mimic 组的克隆形成率、 侵袭细胞数目及 PTBP1 表达明显下降, 而 miR-133b inhibitor 组上升; 与 miR-133b mimic + pc-NC 组比较, miR-133b mimic + pc-PTBP1 组的克隆形成率、 侵袭细胞数目及 PTBP1 表达水平上升 (P< 0. 05)。 结论 miR-133b 在 RCC 癌组织及细胞中表达下调, 且其可能通过调控 PTBP1 的表达水平来影响细胞的增殖和侵袭能力。

Effect of miR-133b on the Cell Proliferation and Invasion of RenalCell Carcinoma by Targeting PTBP1

Objective To explore the effect of miR-133b on the cell proliferation and invasionof renal cell carcinoma ( RCC) by targeting polypyrimidine tract binding protein 1 ( PTBP1 ). Methods The expression level of miR-133b in RCC tissues and cells were detected. The targetingrelationship between miR-133b and PTBP1 was verified. The effect of miR-133b on proliferation andmigration of RCC cells were detected. Results The expression level of miR-133b in RCC tissueswas decreased, while PTBP1 was up-regulated when compared with para-carcinoma tissues (P <0. 05). PTBP1was the target gene of miR-133b. The colony formation rate, the number of invasivecells and the expression level of PTBP1 were significantly decreased in miR-133b mimic group, butwere significantly increased in miR-133b inhibitor group, when compared with miR-NC group. Thecolony formation rate, the number of invasive cells and the expression level of PTBP1 were increased in miR-133b mimic + pc-PTBP1 group when compared with miR-133b mimic + pc-NC group(P< 0. 05). Conclusion miR-133b expression is down-regulated in RCC tissues and cells, whichmay affect cell proliferation and invasion by the regulation of PTBP1.