SOX9在SD大鼠胚胎发育髁突软骨与胫骨生长板软骨中的时间表达研究

目的 通过建立SD大鼠的发育模型,观察髁突软骨与胫骨生长板软骨在胚胎发育过程中的不同生长特性,探讨比较SOX9在2种不同性质软骨生长发育过程中的表达变化及其意义.方法 30只成年SD大鼠按2雌1雄的比例合笼,观察雌鼠于合笼后是否发现阴栓作为怀孕的标志,计为妊娠0d,隔离常规饲养.分别于孕鼠妊娠13d、14 d、15d、...

The Spation-temporal Expression of SOX9 during the Development of Mandibular Condylar Cartilage and Tibial Growth Plate Cartilage in SD Rats

  Objective  To explore the spation-temporal expression changes of SOX9during the development of condylar cartilage and tibial growth plate cartilage in SD rats.   Methods  Thirty adult SD rats were caged at the female-male ratio of 2∶1. The vaginal plug appeared was designated as gestation day 0 （GD0） and the rats were isolated then and raised in the routine way. The embryos were obtained from day 13 to day 21 respectively and the condylar cartilage and tibial growth plate cartilage were harvested. Serial section was performed to obtain the slices of the two kinds of cartilages at different development stages （E13 d-E21 d）. HE staining and immunohistochemical detection were employed to compare the structure distinguish between two kinds of cartilages and examine the expressional changes of SOX9. The data were analyzed by applying single factor analysis of variance and two independent sample t test in SPSS17.0 software package.   Results  Some morphological differences, mainly in the forms, stratification and arrangements of cells, were observed between the condylar cartilage and the tibial growth plate cartilage during E14 d-E21 d indicated by HE staining. In tibial growth plate cartilage, the SOX9 positive expression in E15 d, E17 d and E20 d were stronger than those in condylar cartilage （P < 0.05）. The distribution of positive cells in tibial growth plate and cartilage condylar cartilage was basically identical. SOX9 was mainly positive in the proliferation layer of the two kinds of cartilage.   Conclusions  The main structures of condylar cartilage and tibial growth plate cartilage during the development process are similar. The minority distinguished part is the layered form, which indicates different growth characteristics during embryonic development. During embryonic development, SOX9 is expressed in the two kinds of cartilages above and mainly distributes in the proliferative zone, which shows SOX9 is involved in the cell proliferation and differentiation associated with the differentiation of cartilage cell and development of cartilage.