促渗剂对八桂止痛乳膏体外经皮吸收的影响

  目的  研究八桂止痛乳膏的最佳制备方法，确定最佳基质配方，考察不同浓度促渗剂氮酮对八桂止痛乳膏体外透皮吸收的影响。  方法  制备含不同浓度促渗剂的八桂止痛乳膏。采用 Franz扩散池法，离体小鼠皮肤为经皮吸收材料，高效液相色谱法作为此研究测定方法，比较桂皮醛在不同浓度促渗剂下的累积渗透量（Q）、经皮吸收速率（J），以此为指标，筛选出最佳促渗剂浓度。  结果  不同浓度的氮酮均不同程度地促进桂皮醛体外渗透，其促渗顺序为: 5%氮酮组 > 3%氮酮组 > 7%氮酮组 > 1%氮酮组 > 10%氮酮组。经多重比较，差异均有统计学意义（P < 0.05）。  结论  在含1%、3%、5%、7%和10%氮酮的八桂止痛乳膏中，选择5%的氮酮作为八桂止痛乳膏的促渗剂最佳。

Effects of Different Penetration Enhancers on in vitro Transdermal Permeation of Bagui Zhitong Cream

  Objective  To study the best preparation method of Bagui Zhitong cream, to determine the best matrix formulation, and to investigate the effect of different Penetration Enhancers on in vitro Transdermal Permeation of Bagui Zhitong Cream.   Methods  We prepared Bagui Zhitong Cream with different concentrations of penetration enhancer of azone. Modified Franz diffusion cell was adopted using mouse skin of isolated male rat as transdermal barrier. The content of Cinnamaldehyde was determined by UPLC. The accumulative transdermal amount （Q）and percutaneous permeability （J）were calculated and compared between different concentrations of BaGui Zhitong cream to select the best concentration of Penetration Enhancers.   Results   Different concentrations of azone promoted Cinnamaldehyde in vitro permeation in different degrees, the order of promoting infiltration was as follows: 5% azone > 3% azone > 7% azone > 1% azone > 10% azone. Through the multiple comparison, the difference had statistical significance （P < 0.05）.   Conclusion  In Bagui Zhitong cream containing 1% azone, 3% azone, 5% azone, 7% azone and 10% azone, 5% azone as penetration enhancer has the best effect in Bagui Zhitong Cream.